Cryptosporidiosis in two alpaca (Lama pacos) holdings in the South-West of England

D. F. Twomey, A. M. Barlow, S. Bell, R. M. Chalmers, K. Elwin, M. Giles, Robert Higgins, G. Robinson, R. M. Stringer

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Cryptosporidiosis was investigated on two alpaca (Lama pacos) holdings in the South-West of England. Diagnosis was initially confirmed in a cria with diarrhoea from each holding. Cohort faeces samples were subsequently collected and examined for presence of Cryptosporidium oocysts by immunofluorescence microscopy. On the first holding, 30 samples (24 adults, 6 crias) were tested, and oocysts were detected in three of the cria samples but in none of the adults. On the second holding, 14 floor faeces samples representing apparently healthy crias and one faeces sample from a cria with diarrhoea were collected. Oocysts were detected in four of the "healthy" faeces samples and the sample of diarrhoeic faeces. All isolates were confirmed as Cryptosporidium parvum using polymerase chain reaction restriction fragment length polymorphism of the cryptosporidium oocyst wall protein (COWP) and ssu rRNA genes. Sequence analysis of a 741 bp region of ssu rDNA was carried out on nine of these and revealed high sequence homology with previously reported C. parvum isolates. This investigation highlights the possibility of alpaca crias subclinically shedding oocysts, which has implications for epidemiology and transmission in animals as well as raising zoonotic concerns for human contacts. Gene sequencing of UK isolates from South American camelids is also described for the first time.

Original languageEnglish (US)
Pages (from-to)419-422
Number of pages4
JournalVeterinary Journal
Volume175
Issue number3
DOIs
StatePublished - Mar 1 2008
Externally publishedYes

Fingerprint

New World Camelids
Cryptosporidiosis
cryptosporidiosis
alpacas
Feces
England
Oocysts
oocysts
feces
Cryptosporidium parvum
Diarrhea
sampling
Cryptosporidium
diarrhea
Zoonoses
Sequence Homology
Ribosomal DNA
rRNA Genes
Fluorescence Microscopy
Restriction Fragment Length Polymorphisms

Keywords

  • Alpaca
  • Cria
  • Cryptosporidiosis
  • Cryptosporidium parvum
  • Diarrhoea
  • South American camelid

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Twomey, D. F., Barlow, A. M., Bell, S., Chalmers, R. M., Elwin, K., Giles, M., ... Stringer, R. M. (2008). Cryptosporidiosis in two alpaca (Lama pacos) holdings in the South-West of England. Veterinary Journal, 175(3), 419-422. https://doi.org/10.1016/j.tvjl.2007.01.017

Cryptosporidiosis in two alpaca (Lama pacos) holdings in the South-West of England. / Twomey, D. F.; Barlow, A. M.; Bell, S.; Chalmers, R. M.; Elwin, K.; Giles, M.; Higgins, Robert; Robinson, G.; Stringer, R. M.

In: Veterinary Journal, Vol. 175, No. 3, 01.03.2008, p. 419-422.

Research output: Contribution to journalArticle

Twomey, DF, Barlow, AM, Bell, S, Chalmers, RM, Elwin, K, Giles, M, Higgins, R, Robinson, G & Stringer, RM 2008, 'Cryptosporidiosis in two alpaca (Lama pacos) holdings in the South-West of England', Veterinary Journal, vol. 175, no. 3, pp. 419-422. https://doi.org/10.1016/j.tvjl.2007.01.017
Twomey DF, Barlow AM, Bell S, Chalmers RM, Elwin K, Giles M et al. Cryptosporidiosis in two alpaca (Lama pacos) holdings in the South-West of England. Veterinary Journal. 2008 Mar 1;175(3):419-422. https://doi.org/10.1016/j.tvjl.2007.01.017
Twomey, D. F. ; Barlow, A. M. ; Bell, S. ; Chalmers, R. M. ; Elwin, K. ; Giles, M. ; Higgins, Robert ; Robinson, G. ; Stringer, R. M. / Cryptosporidiosis in two alpaca (Lama pacos) holdings in the South-West of England. In: Veterinary Journal. 2008 ; Vol. 175, No. 3. pp. 419-422.
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