Cryptococcus neoformans promotes its transmigration into the central nervous system by inducing molecular and cellular changes in brain endothelial cells

Kiem Vu, Richard A. Eigenheer, Brett S. Phinney, Angela C Gelli

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Cryptococcus spp. cause fungal meningitis, a life-threatening infection that occurs predominately in immunocompromised individuals. In order for Cryptococcus neoformans to invade the central nervous system (CNS), it must first penetrate the brain endothelium, also known as the blood-brain barrier (BBB). Despite the importance of the interrelation between C. neoformans and the brain endothelium in establishing CNS infection, very little is known about this microenvironment. Here we sought to resolve the cellular and molecular basis that defines the fungal-BBB interface during cryptococcal attachment to, and internalization by, the human brain endothelium. In order to accomplish this by a systems-wide approach, the proteomic profile of human brain endothelial cells challenged with C. neoformans was resolved using a label-free differential quantitative mass spectrometry method known as spectral counting (SC). Here, we demonstrate that as brain endothelial cells associate with, and internalize, cryptococci, they upregulate the expression of several proteins involved with cytoskeleton, metabolism, signaling, and inflammation, suggesting that they are actively signaling and undergoing cytoskeleton remodeling via annexin A2, S100A10, transgelin, and myosin. Transmission electronic microscopy (TEM) analysis demonstrates dramatic structural changes in nuclei, mitochondria, the endoplasmic reticulum (ER), and the plasma membrane that are indicative of cell stress and cell damage. The translocation of HMGB1, a marker of cell injury, the downregulation of proteins that function in transcription, energy production, protein processing, and the upregulation of cyclophilin A further support the notion that C. neoformans elicits changes in brain endothelial cells that facilitate the migration of cryptococci across the BBB and ultimately induce endothelial cell necrosis.

Original languageEnglish (US)
Pages (from-to)3139-3147
Number of pages9
JournalInfection and Immunity
Volume81
Issue number9
DOIs
StatePublished - 2013

Fingerprint

Cryptococcus neoformans
Central Nervous System
Endothelial Cells
Cryptococcus
Brain
Blood-Brain Barrier
Endothelium
Cytoskeleton
Fungal Meningitis
Up-Regulation
Cyclophilin A
Annexin A2
HMGB1 Protein
Central Nervous System Infections
Proteins
Myosins
Endoplasmic Reticulum
Proteomics
Cell Movement
Microscopy

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Cryptococcus neoformans promotes its transmigration into the central nervous system by inducing molecular and cellular changes in brain endothelial cells. / Vu, Kiem; Eigenheer, Richard A.; Phinney, Brett S.; Gelli, Angela C.

In: Infection and Immunity, Vol. 81, No. 9, 2013, p. 3139-3147.

Research output: Contribution to journalArticle

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