Cryostorage and oxidative stress in mammalian spermatozoa

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Citations (Scopus)

Abstract

Although cryopreservation of ejaculated sperm has been in clinical and agricultural use for decades, it is not completely clear how the damage that sperm incur as a result of cryopreservation contributes to fertilization failure, or embryonic or fetal loss. Oxygen is required for life, but oxidative metabolism, particularly during low temperature storage, of biological molecules can be potentially toxic due to the formation of reactive oxygen species (ROS) that can modify cell functions or viability. A limited ability to store antioxidant enzymes combined with a membrane rich in unsaturated fatty acids makes spermatozoa particularly susceptible to oxidative stress and peroxidative attack by ROS, specifically superoxide anion and hydrogen peroxide. This chapter outlines the primary mechanisms of sperm damage during cryopreservation and loss of subsequent fertility and discusses the potential mechanisms of DNA/chromosomal fragmentation and damage, lipid peroxidation, and intracellular ice formation and associated cell damage. The origin of ROS in sperm is discussed as well as how ROS are processed and ultimately scavenged by sperm.

Original languageEnglish (US)
Title of host publicationStudies on Men's Health and Fertility
PublisherHumana Press Inc.
Pages41-56
Number of pages16
ISBN (Electronic)9781617797767
ISBN (Print)9781617797750
DOIs
StatePublished - Jan 1 2012

Fingerprint

Oxidative stress
Spermatozoa
Reactive Oxygen Species
Oxidative Stress
Cryopreservation
Poisons
Ice
Unsaturated Fatty Acids
Metabolism
Superoxides
Hydrogen Peroxide
Antioxidants
Cells
DNA Fragmentation
Oxygen
Membranes
Lipids
Fertilization
Lipid Peroxidation
DNA Damage

Keywords

  • Cryopreservation effects
  • Cryostorage
  • Mammalian spermatozoa
  • Origin of reactive oxygen species
  • Oxidative stress
  • Semen
  • Sperm
  • Sperm fertility

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Meyers, S. A. (2012). Cryostorage and oxidative stress in mammalian spermatozoa. In Studies on Men's Health and Fertility (pp. 41-56). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-776-7_3

Cryostorage and oxidative stress in mammalian spermatozoa. / Meyers, Stuart A.

Studies on Men's Health and Fertility. Humana Press Inc., 2012. p. 41-56.

Research output: Chapter in Book/Report/Conference proceedingChapter

Meyers, SA 2012, Cryostorage and oxidative stress in mammalian spermatozoa. in Studies on Men's Health and Fertility. Humana Press Inc., pp. 41-56. https://doi.org/10.1007/978-1-61779-776-7_3
Meyers SA. Cryostorage and oxidative stress in mammalian spermatozoa. In Studies on Men's Health and Fertility. Humana Press Inc. 2012. p. 41-56 https://doi.org/10.1007/978-1-61779-776-7_3
Meyers, Stuart A. / Cryostorage and oxidative stress in mammalian spermatozoa. Studies on Men's Health and Fertility. Humana Press Inc., 2012. pp. 41-56
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