Cryopreservation of spermatozoa from cynomolgus monkeys (Macaca fascicularis)

Theodore L Tollner, C. A. VandeVoort, J. W. Overstreet, E. Z. Drobnis

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Three egg-yolk diluents, which have been used successfully in cryopreservation of human spermatozoa, were compared for their ability to protect macaque semen against cryodamage. TEST (Tes + Tris + egg yolk), TEST with 20% skim milk (TSM), and egg yolk-citrate (EYC), each with 3 or 5% glycerol were compared using 12 ejaculates from 6 male cynomolgus macaques. Computed-aided analysis of sperm motion was used to determine the percentage motility (%M), curvilinear velocity (VCL), and linearity (LIN) of spermatozoa after thawing. The supravital stain Hoechst 33258 and a fluoresceinated pea lectin were used to determine the % of viable spermatozoa with intact acrosomes. TSM and TEST were superior to EYC in terms of %M and of % viable, acrosome-intact spermatozoa. TSM and TEST produced equivalent VCL and LIN values, while EYC had clearly reduced VCL and LIN. There were no interactions between diluent and glycerol level. The 3% glycerol level gave superior results to 5% glycerol for %M. EYC, which is widely used for cryopreservation of human spermatozoa, was not suitable for cynomolgus monkey semen. Artificial insemination with semen cryopreserved in TSM resulted in a healthy, full-term infant.

Original languageEnglish (US)
Pages (from-to)347-352
Number of pages6
JournalJournal of Reproduction and Fertility
Volume90
Issue number2
StatePublished - 1990

Fingerprint

Egg Yolk
Macaca fascicularis
Cryopreservation
Spermatozoa
Citric Acid
Glycerol
Milk
Semen
Acrosome
Macaca
Bisbenzimidazole
Artificial Insemination
Coloring Agents

Keywords

  • Acrosome
  • Cryopreservation
  • Macaques
  • Motility
  • Spermatozoa

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Embryology
  • Developmental Biology
  • Molecular Biology
  • Physiology

Cite this

Cryopreservation of spermatozoa from cynomolgus monkeys (Macaca fascicularis). / Tollner, Theodore L; VandeVoort, C. A.; Overstreet, J. W.; Drobnis, E. Z.

In: Journal of Reproduction and Fertility, Vol. 90, No. 2, 1990, p. 347-352.

Research output: Contribution to journalArticle

Tollner, TL, VandeVoort, CA, Overstreet, JW & Drobnis, EZ 1990, 'Cryopreservation of spermatozoa from cynomolgus monkeys (Macaca fascicularis)', Journal of Reproduction and Fertility, vol. 90, no. 2, pp. 347-352.
Tollner, Theodore L ; VandeVoort, C. A. ; Overstreet, J. W. ; Drobnis, E. Z. / Cryopreservation of spermatozoa from cynomolgus monkeys (Macaca fascicularis). In: Journal of Reproduction and Fertility. 1990 ; Vol. 90, No. 2. pp. 347-352.
@article{bb07f3b3f8694f21a040f3e26b0f00e1,
title = "Cryopreservation of spermatozoa from cynomolgus monkeys (Macaca fascicularis)",
abstract = "Three egg-yolk diluents, which have been used successfully in cryopreservation of human spermatozoa, were compared for their ability to protect macaque semen against cryodamage. TEST (Tes + Tris + egg yolk), TEST with 20{\%} skim milk (TSM), and egg yolk-citrate (EYC), each with 3 or 5{\%} glycerol were compared using 12 ejaculates from 6 male cynomolgus macaques. Computed-aided analysis of sperm motion was used to determine the percentage motility ({\%}M), curvilinear velocity (VCL), and linearity (LIN) of spermatozoa after thawing. The supravital stain Hoechst 33258 and a fluoresceinated pea lectin were used to determine the {\%} of viable spermatozoa with intact acrosomes. TSM and TEST were superior to EYC in terms of {\%}M and of {\%} viable, acrosome-intact spermatozoa. TSM and TEST produced equivalent VCL and LIN values, while EYC had clearly reduced VCL and LIN. There were no interactions between diluent and glycerol level. The 3{\%} glycerol level gave superior results to 5{\%} glycerol for {\%}M. EYC, which is widely used for cryopreservation of human spermatozoa, was not suitable for cynomolgus monkey semen. Artificial insemination with semen cryopreserved in TSM resulted in a healthy, full-term infant.",
keywords = "Acrosome, Cryopreservation, Macaques, Motility, Spermatozoa",
author = "Tollner, {Theodore L} and VandeVoort, {C. A.} and Overstreet, {J. W.} and Drobnis, {E. Z.}",
year = "1990",
language = "English (US)",
volume = "90",
pages = "347--352",
journal = "Journal of Reproduction and Fertility",
issn = "0022-4251",
publisher = "Society for Reproduction and Fertility",
number = "2",

}

TY - JOUR

T1 - Cryopreservation of spermatozoa from cynomolgus monkeys (Macaca fascicularis)

AU - Tollner, Theodore L

AU - VandeVoort, C. A.

AU - Overstreet, J. W.

AU - Drobnis, E. Z.

PY - 1990

Y1 - 1990

N2 - Three egg-yolk diluents, which have been used successfully in cryopreservation of human spermatozoa, were compared for their ability to protect macaque semen against cryodamage. TEST (Tes + Tris + egg yolk), TEST with 20% skim milk (TSM), and egg yolk-citrate (EYC), each with 3 or 5% glycerol were compared using 12 ejaculates from 6 male cynomolgus macaques. Computed-aided analysis of sperm motion was used to determine the percentage motility (%M), curvilinear velocity (VCL), and linearity (LIN) of spermatozoa after thawing. The supravital stain Hoechst 33258 and a fluoresceinated pea lectin were used to determine the % of viable spermatozoa with intact acrosomes. TSM and TEST were superior to EYC in terms of %M and of % viable, acrosome-intact spermatozoa. TSM and TEST produced equivalent VCL and LIN values, while EYC had clearly reduced VCL and LIN. There were no interactions between diluent and glycerol level. The 3% glycerol level gave superior results to 5% glycerol for %M. EYC, which is widely used for cryopreservation of human spermatozoa, was not suitable for cynomolgus monkey semen. Artificial insemination with semen cryopreserved in TSM resulted in a healthy, full-term infant.

AB - Three egg-yolk diluents, which have been used successfully in cryopreservation of human spermatozoa, were compared for their ability to protect macaque semen against cryodamage. TEST (Tes + Tris + egg yolk), TEST with 20% skim milk (TSM), and egg yolk-citrate (EYC), each with 3 or 5% glycerol were compared using 12 ejaculates from 6 male cynomolgus macaques. Computed-aided analysis of sperm motion was used to determine the percentage motility (%M), curvilinear velocity (VCL), and linearity (LIN) of spermatozoa after thawing. The supravital stain Hoechst 33258 and a fluoresceinated pea lectin were used to determine the % of viable spermatozoa with intact acrosomes. TSM and TEST were superior to EYC in terms of %M and of % viable, acrosome-intact spermatozoa. TSM and TEST produced equivalent VCL and LIN values, while EYC had clearly reduced VCL and LIN. There were no interactions between diluent and glycerol level. The 3% glycerol level gave superior results to 5% glycerol for %M. EYC, which is widely used for cryopreservation of human spermatozoa, was not suitable for cynomolgus monkey semen. Artificial insemination with semen cryopreserved in TSM resulted in a healthy, full-term infant.

KW - Acrosome

KW - Cryopreservation

KW - Macaques

KW - Motility

KW - Spermatozoa

UR - http://www.scopus.com/inward/record.url?scp=0025080931&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025080931&partnerID=8YFLogxK

M3 - Article

C2 - 2250234

AN - SCOPUS:0025080931

VL - 90

SP - 347

EP - 352

JO - Journal of Reproduction and Fertility

JF - Journal of Reproduction and Fertility

SN - 0022-4251

IS - 2

ER -