Abstract
Three egg-yolk diluents, which have been used successfully in cryopreservation of human spermatozoa, were compared for their ability to protect macaque semen against cryodamage. TEST (Tes + Tris + egg yolk), TEST with 20% skim milk (TSM), and egg yolk-citrate (EYC), each with 3 or 5% glycerol were compared using 12 ejaculates from 6 male cynomolgus macaques. Computed-aided analysis of sperm motion was used to determine the percentage motility (%M), curvilinear velocity (VCL), and linearity (LIN) of spermatozoa after thawing. The supravital stain Hoechst 33258 and a fluoresceinated pea lectin were used to determine the % of viable spermatozoa with intact acrosomes. TSM and TEST were superior to EYC in terms of %M and of % viable, acrosome-intact spermatozoa. TSM and TEST produced equivalent VCL and LIN values, while EYC had clearly reduced VCL and LIN. There were no interactions between diluent and glycerol level. The 3% glycerol level gave superior results to 5% glycerol for %M. EYC, which is widely used for cryopreservation of human spermatozoa, was not suitable for cynomolgus monkey semen. Artificial insemination with semen cryopreserved in TSM resulted in a healthy, full-term infant.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 347-352 |
| Number of pages | 6 |
| Journal | Journal of Reproduction and Fertility |
| Volume | 90 |
| Issue number | 2 |
| State | Published - 1990 |
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Keywords
- Acrosome
- Cryopreservation
- Macaques
- Motility
- Spermatozoa
ASJC Scopus subject areas
- Obstetrics and Gynecology
- Embryology
- Developmental Biology
- Molecular Biology
- Physiology
Cite this
Cryopreservation of spermatozoa from cynomolgus monkeys (Macaca fascicularis). / Tollner, Theodore L; VandeVoort, C. A.; Overstreet, J. W.; Drobnis, E. Z.
In: Journal of Reproduction and Fertility, Vol. 90, No. 2, 1990, p. 347-352.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cryopreservation of spermatozoa from cynomolgus monkeys (Macaca fascicularis)
AU - Tollner, Theodore L
AU - VandeVoort, C. A.
AU - Overstreet, J. W.
AU - Drobnis, E. Z.
PY - 1990
Y1 - 1990
N2 - Three egg-yolk diluents, which have been used successfully in cryopreservation of human spermatozoa, were compared for their ability to protect macaque semen against cryodamage. TEST (Tes + Tris + egg yolk), TEST with 20% skim milk (TSM), and egg yolk-citrate (EYC), each with 3 or 5% glycerol were compared using 12 ejaculates from 6 male cynomolgus macaques. Computed-aided analysis of sperm motion was used to determine the percentage motility (%M), curvilinear velocity (VCL), and linearity (LIN) of spermatozoa after thawing. The supravital stain Hoechst 33258 and a fluoresceinated pea lectin were used to determine the % of viable spermatozoa with intact acrosomes. TSM and TEST were superior to EYC in terms of %M and of % viable, acrosome-intact spermatozoa. TSM and TEST produced equivalent VCL and LIN values, while EYC had clearly reduced VCL and LIN. There were no interactions between diluent and glycerol level. The 3% glycerol level gave superior results to 5% glycerol for %M. EYC, which is widely used for cryopreservation of human spermatozoa, was not suitable for cynomolgus monkey semen. Artificial insemination with semen cryopreserved in TSM resulted in a healthy, full-term infant.
AB - Three egg-yolk diluents, which have been used successfully in cryopreservation of human spermatozoa, were compared for their ability to protect macaque semen against cryodamage. TEST (Tes + Tris + egg yolk), TEST with 20% skim milk (TSM), and egg yolk-citrate (EYC), each with 3 or 5% glycerol were compared using 12 ejaculates from 6 male cynomolgus macaques. Computed-aided analysis of sperm motion was used to determine the percentage motility (%M), curvilinear velocity (VCL), and linearity (LIN) of spermatozoa after thawing. The supravital stain Hoechst 33258 and a fluoresceinated pea lectin were used to determine the % of viable spermatozoa with intact acrosomes. TSM and TEST were superior to EYC in terms of %M and of % viable, acrosome-intact spermatozoa. TSM and TEST produced equivalent VCL and LIN values, while EYC had clearly reduced VCL and LIN. There were no interactions between diluent and glycerol level. The 3% glycerol level gave superior results to 5% glycerol for %M. EYC, which is widely used for cryopreservation of human spermatozoa, was not suitable for cynomolgus monkey semen. Artificial insemination with semen cryopreserved in TSM resulted in a healthy, full-term infant.
KW - Acrosome
KW - Cryopreservation
KW - Macaques
KW - Motility
KW - Spermatozoa
UR - http://www.scopus.com/inward/record.url?scp=0025080931&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025080931&partnerID=8YFLogxK
M3 - Article
C2 - 2250234
AN - SCOPUS:0025080931
VL - 90
SP - 347
EP - 352
JO - Journal of Reproduction and Fertility
JF - Journal of Reproduction and Fertility
SN - 0022-4251
IS - 2
ER -