Cryopreservation of autologous fat grafts harvested with the coleman technique

Lee Li-Qun Pu, Sydney R. Coleman, Xiangdong Cui, Robert E H Ferguson, Henry C. Vasconez

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The viability of fat grafts harvested with an established technique after cryopreservation remains unknown. This study was conducted in vitro to evaluate the viability of autologous fat grafts harvested with the Coleman technique and subsequently preserved with our preferred cryopreservation method. Eight adult females were enrolled in this study. In each patient, 10 mL of fat grafts were harvested with the Coleman technique by a single surgeon from the lower abdomen. In group 1, 5 mL of fresh fat grafts were mixed with cryoprotective agents and underwent cryopreservation with controlled slow cooling and fast rewarming. In group 2, 5 mL of fresh fat grafts without cryopreservation from the same patient served as a control. The fat graft samples from both groups were evaluated with trypan blue vital staining, glycerol-3-phophatase dehydrogenase assay, and routine histology. Viable adipocyte counts were found similar in both group 1 and group 2 (3.46 ± 0.91 vs. 4.12 ± 1.11 × 10 6/mL, P = 0.22). However, glycerol-3-phophatase dehydrogenase activity was significantly lower in group 1 compared with group 2 (0.47 ± 0.09 vs. 0.66 ± 0.09 u/mL, P < 0.001). Histologically, the normal structure of fragmented fatty tissues was found primarily in both groups. Our results indicate that autologous fat grafts harvested with the Coleman technique and preserved with our preferred cryopreservation method have a normal histology with near the same number of viable adipocytes as compared with the fresh fat grafts. However, those cryopreserved fat grafts appear to have a less optimal level of adipocyte specific enzyme activity compared with the fresh ones and thus may not survive well after they are transplanted without being optimized.

Original languageEnglish (US)
Pages (from-to)333-337
Number of pages5
JournalAnnals of Plastic Surgery
Volume64
Issue number3
DOIs
StatePublished - Mar 2010

Fingerprint

Cryopreservation
Fats
Transplants
Adipocytes
Glycerol
Histology
Oxidoreductases
Cryoprotective Agents
Rewarming
Trypan Blue
Abdomen
Adipose Tissue
Staining and Labeling
Enzymes

Keywords

  • Coleman technique
  • Cryopreservation
  • Fat grafts
  • Viability

ASJC Scopus subject areas

  • Surgery

Cite this

Cryopreservation of autologous fat grafts harvested with the coleman technique. / Pu, Lee Li-Qun; Coleman, Sydney R.; Cui, Xiangdong; Ferguson, Robert E H; Vasconez, Henry C.

In: Annals of Plastic Surgery, Vol. 64, No. 3, 03.2010, p. 333-337.

Research output: Contribution to journalArticle

Pu, Lee Li-Qun ; Coleman, Sydney R. ; Cui, Xiangdong ; Ferguson, Robert E H ; Vasconez, Henry C. / Cryopreservation of autologous fat grafts harvested with the coleman technique. In: Annals of Plastic Surgery. 2010 ; Vol. 64, No. 3. pp. 333-337.
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