Cryopreparation and electron tomography of yeast cells

Eileen T. O’Toole; Thomas H. Giddings; Mark Winey

doi:10.1101/pdb.prot085589

Cryopreparation and electron tomography of yeast cells

Eileen T. O’Toole, Thomas H. Giddings, Mark Winey

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Three-dimensional imaging of cells using electron tomography enables analysis of cell structure at unprecedented resolution. The preparation of cells for tomography using rapid freezing followed by freeze-substitution is an essential first step to ensure the optimal preservation of the cell structure for 3D studies. This protocol outlines a method for obtaining well-preserved cells using high-pressure freezing followed by freeze-substitution. We have found that this method is particularly well suited for electron tomography studies and has the added bonus of preserving antigenicity for immuno-electron microscopy. The steps involved in imaging cells and performing tomographic analysis of cellular structures are also outlined.

Original language	English (US)
Pages (from-to)	212-216
Number of pages	5
Journal	Cold Spring Harbor Protocols
Volume	2017
Issue number	3
DOIs	https://doi.org/10.1101/pdb.prot085589
State	Published - Mar 1 2017
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1101/pdb.prot085589

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Cryopreparation and electron tomography of yeast cells

Abstract

ASJC Scopus subject areas

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