Crossover Homeostasis in Yeast Meiosis

Emmanuelle Martini, Robert L. Diaz, Neil Hunter, Scott Keeney

Research output: Contribution to journalArticle

196 Citations (Scopus)

Abstract

Crossovers produced by homologous recombination promote accurate chromosome segregation in meiosis and are controlled such that at least one forms per chromosome pair and multiple crossovers are widely spaced. Recombination initiates with an excess number of double-strand breaks made by Spo11 protein. Thus, crossover control involves a decision by which some breaks give crossovers while others follow a predominantly noncrossover pathway(s). To understand this decision, we examined recombination when breaks are reduced in yeast spo11 hypomorphs. We find that crossover levels tend to be maintained at the expense of noncrossovers and that genomic loci differ in expression of this "crossover homeostasis." These findings define a previously unsuspected manifestation of crossover control, i.e., that the crossover/noncrossover ratio can change to maintain crossovers. Our results distinguish between existing models of crossover control and support the hypothesis that an obligate crossover is a genetically programmed event tied to crossover interference.

Original languageEnglish (US)
Pages (from-to)285-295
Number of pages11
JournalCell
Volume126
Issue number2
DOIs
StatePublished - Jul 28 2006

Fingerprint

Meiosis
Yeast
Genetic Recombination
Homeostasis
Yeasts
Chromosomes
Chromosome Segregation
Homologous Recombination
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Martini, E., Diaz, R. L., Hunter, N., & Keeney, S. (2006). Crossover Homeostasis in Yeast Meiosis. Cell, 126(2), 285-295. https://doi.org/10.1016/j.cell.2006.05.044

Crossover Homeostasis in Yeast Meiosis. / Martini, Emmanuelle; Diaz, Robert L.; Hunter, Neil; Keeney, Scott.

In: Cell, Vol. 126, No. 2, 28.07.2006, p. 285-295.

Research output: Contribution to journalArticle

Martini, E, Diaz, RL, Hunter, N & Keeney, S 2006, 'Crossover Homeostasis in Yeast Meiosis', Cell, vol. 126, no. 2, pp. 285-295. https://doi.org/10.1016/j.cell.2006.05.044
Martini, Emmanuelle ; Diaz, Robert L. ; Hunter, Neil ; Keeney, Scott. / Crossover Homeostasis in Yeast Meiosis. In: Cell. 2006 ; Vol. 126, No. 2. pp. 285-295.
@article{9478dcef980e4c458c68ba24eb095646,
title = "Crossover Homeostasis in Yeast Meiosis",
abstract = "Crossovers produced by homologous recombination promote accurate chromosome segregation in meiosis and are controlled such that at least one forms per chromosome pair and multiple crossovers are widely spaced. Recombination initiates with an excess number of double-strand breaks made by Spo11 protein. Thus, crossover control involves a decision by which some breaks give crossovers while others follow a predominantly noncrossover pathway(s). To understand this decision, we examined recombination when breaks are reduced in yeast spo11 hypomorphs. We find that crossover levels tend to be maintained at the expense of noncrossovers and that genomic loci differ in expression of this {"}crossover homeostasis.{"} These findings define a previously unsuspected manifestation of crossover control, i.e., that the crossover/noncrossover ratio can change to maintain crossovers. Our results distinguish between existing models of crossover control and support the hypothesis that an obligate crossover is a genetically programmed event tied to crossover interference.",
author = "Emmanuelle Martini and Diaz, {Robert L.} and Neil Hunter and Scott Keeney",
year = "2006",
month = "7",
day = "28",
doi = "10.1016/j.cell.2006.05.044",
language = "English (US)",
volume = "126",
pages = "285--295",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "2",

}

TY - JOUR

T1 - Crossover Homeostasis in Yeast Meiosis

AU - Martini, Emmanuelle

AU - Diaz, Robert L.

AU - Hunter, Neil

AU - Keeney, Scott

PY - 2006/7/28

Y1 - 2006/7/28

N2 - Crossovers produced by homologous recombination promote accurate chromosome segregation in meiosis and are controlled such that at least one forms per chromosome pair and multiple crossovers are widely spaced. Recombination initiates with an excess number of double-strand breaks made by Spo11 protein. Thus, crossover control involves a decision by which some breaks give crossovers while others follow a predominantly noncrossover pathway(s). To understand this decision, we examined recombination when breaks are reduced in yeast spo11 hypomorphs. We find that crossover levels tend to be maintained at the expense of noncrossovers and that genomic loci differ in expression of this "crossover homeostasis." These findings define a previously unsuspected manifestation of crossover control, i.e., that the crossover/noncrossover ratio can change to maintain crossovers. Our results distinguish between existing models of crossover control and support the hypothesis that an obligate crossover is a genetically programmed event tied to crossover interference.

AB - Crossovers produced by homologous recombination promote accurate chromosome segregation in meiosis and are controlled such that at least one forms per chromosome pair and multiple crossovers are widely spaced. Recombination initiates with an excess number of double-strand breaks made by Spo11 protein. Thus, crossover control involves a decision by which some breaks give crossovers while others follow a predominantly noncrossover pathway(s). To understand this decision, we examined recombination when breaks are reduced in yeast spo11 hypomorphs. We find that crossover levels tend to be maintained at the expense of noncrossovers and that genomic loci differ in expression of this "crossover homeostasis." These findings define a previously unsuspected manifestation of crossover control, i.e., that the crossover/noncrossover ratio can change to maintain crossovers. Our results distinguish between existing models of crossover control and support the hypothesis that an obligate crossover is a genetically programmed event tied to crossover interference.

UR - http://www.scopus.com/inward/record.url?scp=33746189408&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746189408&partnerID=8YFLogxK

U2 - 10.1016/j.cell.2006.05.044

DO - 10.1016/j.cell.2006.05.044

M3 - Article

VL - 126

SP - 285

EP - 295

JO - Cell

JF - Cell

SN - 0092-8674

IS - 2

ER -