Cross-talk between fibroblast growth factor and bone morphogenetic proteins regulates gap junction-mediated intercellular communication in lens cells

Bruce A. Boswell, Pamela J Lein, Linda S. Musil

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Homeostasis in the lens is dependent on an extensive network of cell-to-cell gap junctional channels. Gap junction-mediated intercellular coupling (GJIC) is higher in the equatorial region of the lens than at either pole, an asymmetry believed essential for lens transparency. Primary cultures of embryonic chick lens epithelial cells up-regulate GJIC in response to purified fibroblast growth factor (FGF)1/2 or to medium conditioned by vitreous bodies, the major reservoir of factors (including FGF) for the lens equator. We show that purified bone morphogenetic protein (BMP)2, -4, and -7 also up-regulate GJIC in these cultures. BMP2, -4, or both are present in vitreous body conditioned medium, and BMP4 and -7 are endogenously expressed by lens cells. Remarkably, lens-derived BMP signaling is required for up-regulation of GJIC by purified FGF, and sufficient for up-regulation by vitreous humor. This is the first demonstration of an obligatory interaction between FGF and BMPs in postplacode lens cells, and of a role for FGF/BMP cross-talk in regulating GJIC in any cell type. Our results support a model in which the angular gradient in GJIC in the lens, and thus proper lens function, is dependent on signaling between the FGF and BMP pathways.

Original languageEnglish (US)
Pages (from-to)2631-2641
Number of pages11
JournalMolecular Biology of the Cell
Volume19
Issue number6
DOIs
StatePublished - Jun 2008
Externally publishedYes

Fingerprint

Bone Morphogenetic Proteins
Fibroblast Growth Factors
Gap Junctions
Lenses
Vitreous Body
Up-Regulation
Conditioned Culture Medium
Bone Morphogenetic Protein 4
Fibroblast Growth Factor 1
Bone Morphogenetic Protein 2
Fibroblast Growth Factor 2
Homeostasis
Epithelial Cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Cross-talk between fibroblast growth factor and bone morphogenetic proteins regulates gap junction-mediated intercellular communication in lens cells. / Boswell, Bruce A.; Lein, Pamela J; Musil, Linda S.

In: Molecular Biology of the Cell, Vol. 19, No. 6, 06.2008, p. 2631-2641.

Research output: Contribution to journalArticle

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