CRL5-dependent regulation of the small GTPases ARL4C and ARF6 controls hippocampal morphogenesis

Jisoo S. Han, Keiko Hino, Wenzhe Li, Raenier V. Reyes, Cesar P. Canales, Adam M. Miltner, Yasmin Haddadi, Junqing Sun, Chao Yin Chen, Anna La Torre, Sergi Simó

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


The small GTPase ARL4C participates in the regulation of cell migration, cytoskeletal rearrangements, and vesicular trafficking in epithelial cells. The ARL4C signaling cascade starts by the recruitment of the ARF–GEF cytohesins to the plasma membrane, which, in turn, bind and activate the small GTPase ARF6. However, the role of ARL4C–cytohesin–ARF6 signaling during hippocampal development remains elusive. Here, we report that the E3 ubiquitin ligase Cullin 5/RBX2 (CRL5) controls the stability of ARL4C and its signaling effectors to regulate hippocampal morphogenesis. Both RBX2 knockout and Cullin 5 knockdown cause hippocampal pyramidal neuron mislocalization and development of multiple apical dendrites. We used quantitative mass spectrometry to show that ARL4C, Cytohesin-1/3, and ARF6 accumulate in the RBX2 mutant telencephalon. Furthermore, we show that depletion of ARL4C rescues the phenotypes caused by Cullin 5 knockdown, whereas depletion of CYTH1 or ARF6 exacerbates overmigration. Finally, we show that ARL4C, CYTH1, and ARF6 are necessary for the dendritic outgrowth of pyramidal neurons to the superficial strata of the hippocampus. Overall, we identified CRL5 as a key regulator of hippocampal development and uncovered ARL4C, CYTH1, and ARF6 as CRL5-regulated signaling effectors that control pyramidal neuron migration and dendritogenesis.

Original languageEnglish (US)
Pages (from-to)23073-23084
Number of pages12
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number37
StatePublished - Sep 15 2020


  • ARF6
  • ARL4C
  • CRL5
  • Hippocampal development
  • Pyramidal neuron migration

ASJC Scopus subject areas

  • General


Dive into the research topics of 'CRL5-dependent regulation of the small GTPases ARL4C and ARF6 controls hippocampal morphogenesis'. Together they form a unique fingerprint.

Cite this