Critical seeding density improves the properties and translatability of self-assembling anatomically shaped knee menisci

Pasha Hadidi, Timothy C. Yeh, Jerry C. Hu, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A recent development in the field of tissue engineering is the rise of all-biologic, scaffold-free engineered tissues. Since these biomaterials rely primarily upon cells, investigation of initial seeding densities constitutes a particularly relevant aim for tissue engineers. In this study, a scaffold-free method was used to create fibrocartilage in the shape of the rabbit knee meniscus. The objectives of this study were to: (i) determine the minimum seeding density, normalized by an area of 44 mm2, necessary for the self-assembling process of fibrocartilage to occur; (ii) examine relevant biomechanical properties of engineered fibrocartilage, such as tensile and compressive stiffness and strength, and their relationship to seeding density; and (iii) identify a reduced, or optimal, number of cells needed to produce this biomaterial. It was found that a decreased initial seeding density, normalized by the area of the construct, produced superior mechanical and biochemical properties. Collagen per wet weight, glycosaminoglycans per wet weight, tensile properties and compressive properties were all significantly greater in the 5 million cells per construct group as compared to the historical 20 million cells per construct group. Scanning electron microscopy demonstrated that a lower seeding density results in a denser tissue. Additionally, the translational potential of the self-assembling process for tissue engineering was improved though this investigation, as fewer cells may be used in the future. The results of this study underscore the potential for critical seeding densities to be investigated when researching scaffold-free engineered tissues.

Original languageEnglish (US)
Pages (from-to)173-182
Number of pages10
JournalActa Biomaterialia
Volume11
Issue number1
DOIs
StatePublished - 2015

Fingerprint

Fibrocartilage
Knee
Scaffolds
Tissue
Biocompatible Materials
Tissue Engineering
Tissue engineering
Biomaterials
Compressive Strength
Weights and Measures
Bioelectric potentials
Glycosaminoglycans
Biological Products
Tensile properties
Collagen
Electron Scanning Microscopy
Cell Count
Stiffness
Rabbits
Engineers

Keywords

  • Biomaterials
  • Biomechanics
  • Knee meniscus
  • Self-assembling process
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Critical seeding density improves the properties and translatability of self-assembling anatomically shaped knee menisci. / Hadidi, Pasha; Yeh, Timothy C.; Hu, Jerry C.; Athanasiou, Kyriacos A.

In: Acta Biomaterialia, Vol. 11, No. 1, 2015, p. 173-182.

Research output: Contribution to journalArticle

Hadidi, Pasha ; Yeh, Timothy C. ; Hu, Jerry C. ; Athanasiou, Kyriacos A. / Critical seeding density improves the properties and translatability of self-assembling anatomically shaped knee menisci. In: Acta Biomaterialia. 2015 ; Vol. 11, No. 1. pp. 173-182.
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