Critical role for galectin-3 in airway inflammation and bronchial hyperresponsiveness in a murine model of asthma

Riaz I. Zuberi, Daniel K. Hsu, Omer Kalayci, Huan Yuan Chen, Holly K. Sheldon, Lan Yu, John R. Apgar, Toshiaki Kawakami, Craig M. Lilly, Fu-Tong Liu

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

Galectin-3 is a member of a β-galactoside-binding animal lectin family. Previous in vitro studies have demonstrated that galectin-3 is involved in a number of activities; however, the roles of this lectin in physiological and pathological processes in vivo remain to be elucidated. Herein, we show, in a murine model of ovalbumin (OVA)-induced asthma that 1) peribronchial inflammatory cells expressed large amounts of galectin-3; 2) bronchoalveolar lavage fluid from OVA-challenged mice contained significantly higher levels of galectin-3 compared to control mice; and 3) macrophages in bronchoalveolar lavage fluid were the major cell type that contained galectin-3. We investigated the role of galectin-3 in the allergic airway response by comparing galectin-3-deficient (gal3-/-) mice and wild-type (gal3 +/+) mice. OVA-sensitized gal3-/- mice developed fewer eosinophils and lower goblet cell metaplasia, after airway OVA challenge compared to similarly treated gal3+/+ mice. In addition, the OVA-sensitized gal3-/- mice developed significantly less airway hyperresponsiveness after airway OVA challenge compared to gal3+/+ mice. Finally, gal3-/- mice developed a lower Th2 response, but a higher Th1 response, suggesting that galectin-3 regulates the Th1/Th2 response. We conclude that galectin-3 may play an important role in the pathogenesis of asthma and inhibitors of this lectin may prove useful for treatment of this disease.

Original languageEnglish (US)
Pages (from-to)2045-2053
Number of pages9
JournalAmerican Journal of Pathology
Volume165
Issue number6
StatePublished - Dec 2004

Fingerprint

Galectin 3
Asthma
Inflammation
Ovalbumin
Lectins
Bronchoalveolar Lavage Fluid
Galectin 2
Physiological Phenomena
Galactosides
Goblet Cells
Metaplasia
Pathologic Processes
Eosinophils

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Zuberi, R. I., Hsu, D. K., Kalayci, O., Chen, H. Y., Sheldon, H. K., Yu, L., ... Liu, F-T. (2004). Critical role for galectin-3 in airway inflammation and bronchial hyperresponsiveness in a murine model of asthma. American Journal of Pathology, 165(6), 2045-2053.

Critical role for galectin-3 in airway inflammation and bronchial hyperresponsiveness in a murine model of asthma. / Zuberi, Riaz I.; Hsu, Daniel K.; Kalayci, Omer; Chen, Huan Yuan; Sheldon, Holly K.; Yu, Lan; Apgar, John R.; Kawakami, Toshiaki; Lilly, Craig M.; Liu, Fu-Tong.

In: American Journal of Pathology, Vol. 165, No. 6, 12.2004, p. 2045-2053.

Research output: Contribution to journalArticle

Zuberi, RI, Hsu, DK, Kalayci, O, Chen, HY, Sheldon, HK, Yu, L, Apgar, JR, Kawakami, T, Lilly, CM & Liu, F-T 2004, 'Critical role for galectin-3 in airway inflammation and bronchial hyperresponsiveness in a murine model of asthma', American Journal of Pathology, vol. 165, no. 6, pp. 2045-2053.
Zuberi, Riaz I. ; Hsu, Daniel K. ; Kalayci, Omer ; Chen, Huan Yuan ; Sheldon, Holly K. ; Yu, Lan ; Apgar, John R. ; Kawakami, Toshiaki ; Lilly, Craig M. ; Liu, Fu-Tong. / Critical role for galectin-3 in airway inflammation and bronchial hyperresponsiveness in a murine model of asthma. In: American Journal of Pathology. 2004 ; Vol. 165, No. 6. pp. 2045-2053.
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