Creation of myelomeningocele in the fetal rabbit

H. Tamiko Housley, Joy L. Graf, Gerald S. Lipshultz, Christopher J. Calvano, Michael R. Harrison, Diana L Farmer, Russel W. Jennings

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Objective: Myelomeningocele is a neural tube defect resulting in an exposed spinal cord, which leads to irreversible neurologic damage at birth. We proposed development of a fetal rabbit model of myelomeningocele to study in utero spinal cord injury and repair strategies. Methods: New Zealand white rabbits (n = 10) at 22 days of gestation (term = 31 days) underwent laparotomy to expose the gravid uterus; a hysterotomy exposed the fetal hindlimbs and back. A three to four level lumbar laminectomy was performed, and the dura over the posterior spinal cord was removed. At 30 days of gestation, the does underwent C-section for fetal harvest, and total fetal number, length, weight, and the presence or absence of a spinal defect were recorded for all viable fetuses. Results: All injured fetuses were smaller and weighed less than the nonoperated littermate controls, and histologic examination confirmed a spina bifida-like lesion of their spinal cords. Conclusions: We successfully created an exposed spinal cord defect in the fetal rabbit model similar to the lesion found in humans. Advantageous because of low animal cost, relatively large fetal size, multiple fetuses per pregnancy, and short total gestation, this model will allow us to study the mechanism of injury to the exposed spinal cord, and perhaps develop strategies to repair human myelomeningoceles. Copyright (C) 2000 S. Karger AG, Basel.

Original languageEnglish (US)
Pages (from-to)275-279
Number of pages5
JournalFetal Diagnosis and Therapy
Volume15
Issue number5
StatePublished - Sep 2000

Fingerprint

Meningomyelocele
Spinal Cord
Rabbits
Pregnancy
Fetus
Hysterotomy
Spinal Cord Regeneration
Spinal Dysraphism
Laminectomy
Neural Tube Defects
Hindlimb
Fetal Development
Spinal Cord Injuries
Laparotomy
Nervous System
Uterus
Parturition
Weights and Measures
Costs and Cost Analysis
Wounds and Injuries

Keywords

  • Birth defects
  • Fetal surgery
  • Myelomeningocele
  • Neural tube defects

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Pediatrics, Perinatology, and Child Health

Cite this

Housley, H. T., Graf, J. L., Lipshultz, G. S., Calvano, C. J., Harrison, M. R., Farmer, D. L., & Jennings, R. W. (2000). Creation of myelomeningocele in the fetal rabbit. Fetal Diagnosis and Therapy, 15(5), 275-279.

Creation of myelomeningocele in the fetal rabbit. / Housley, H. Tamiko; Graf, Joy L.; Lipshultz, Gerald S.; Calvano, Christopher J.; Harrison, Michael R.; Farmer, Diana L; Jennings, Russel W.

In: Fetal Diagnosis and Therapy, Vol. 15, No. 5, 09.2000, p. 275-279.

Research output: Contribution to journalArticle

Housley, HT, Graf, JL, Lipshultz, GS, Calvano, CJ, Harrison, MR, Farmer, DL & Jennings, RW 2000, 'Creation of myelomeningocele in the fetal rabbit', Fetal Diagnosis and Therapy, vol. 15, no. 5, pp. 275-279.
Housley HT, Graf JL, Lipshultz GS, Calvano CJ, Harrison MR, Farmer DL et al. Creation of myelomeningocele in the fetal rabbit. Fetal Diagnosis and Therapy. 2000 Sep;15(5):275-279.
Housley, H. Tamiko ; Graf, Joy L. ; Lipshultz, Gerald S. ; Calvano, Christopher J. ; Harrison, Michael R. ; Farmer, Diana L ; Jennings, Russel W. / Creation of myelomeningocele in the fetal rabbit. In: Fetal Diagnosis and Therapy. 2000 ; Vol. 15, No. 5. pp. 275-279.
@article{6ef88b613a404a78842c5cdb317cbb6d,
title = "Creation of myelomeningocele in the fetal rabbit",
abstract = "Objective: Myelomeningocele is a neural tube defect resulting in an exposed spinal cord, which leads to irreversible neurologic damage at birth. We proposed development of a fetal rabbit model of myelomeningocele to study in utero spinal cord injury and repair strategies. Methods: New Zealand white rabbits (n = 10) at 22 days of gestation (term = 31 days) underwent laparotomy to expose the gravid uterus; a hysterotomy exposed the fetal hindlimbs and back. A three to four level lumbar laminectomy was performed, and the dura over the posterior spinal cord was removed. At 30 days of gestation, the does underwent C-section for fetal harvest, and total fetal number, length, weight, and the presence or absence of a spinal defect were recorded for all viable fetuses. Results: All injured fetuses were smaller and weighed less than the nonoperated littermate controls, and histologic examination confirmed a spina bifida-like lesion of their spinal cords. Conclusions: We successfully created an exposed spinal cord defect in the fetal rabbit model similar to the lesion found in humans. Advantageous because of low animal cost, relatively large fetal size, multiple fetuses per pregnancy, and short total gestation, this model will allow us to study the mechanism of injury to the exposed spinal cord, and perhaps develop strategies to repair human myelomeningoceles. Copyright (C) 2000 S. Karger AG, Basel.",
keywords = "Birth defects, Fetal surgery, Myelomeningocele, Neural tube defects",
author = "Housley, {H. Tamiko} and Graf, {Joy L.} and Lipshultz, {Gerald S.} and Calvano, {Christopher J.} and Harrison, {Michael R.} and Farmer, {Diana L} and Jennings, {Russel W.}",
year = "2000",
month = "9",
language = "English (US)",
volume = "15",
pages = "275--279",
journal = "Fetal Diagnosis and Therapy",
issn = "1015-3837",
publisher = "S. Karger AG",
number = "5",

}

TY - JOUR

T1 - Creation of myelomeningocele in the fetal rabbit

AU - Housley, H. Tamiko

AU - Graf, Joy L.

AU - Lipshultz, Gerald S.

AU - Calvano, Christopher J.

AU - Harrison, Michael R.

AU - Farmer, Diana L

AU - Jennings, Russel W.

PY - 2000/9

Y1 - 2000/9

N2 - Objective: Myelomeningocele is a neural tube defect resulting in an exposed spinal cord, which leads to irreversible neurologic damage at birth. We proposed development of a fetal rabbit model of myelomeningocele to study in utero spinal cord injury and repair strategies. Methods: New Zealand white rabbits (n = 10) at 22 days of gestation (term = 31 days) underwent laparotomy to expose the gravid uterus; a hysterotomy exposed the fetal hindlimbs and back. A three to four level lumbar laminectomy was performed, and the dura over the posterior spinal cord was removed. At 30 days of gestation, the does underwent C-section for fetal harvest, and total fetal number, length, weight, and the presence or absence of a spinal defect were recorded for all viable fetuses. Results: All injured fetuses were smaller and weighed less than the nonoperated littermate controls, and histologic examination confirmed a spina bifida-like lesion of their spinal cords. Conclusions: We successfully created an exposed spinal cord defect in the fetal rabbit model similar to the lesion found in humans. Advantageous because of low animal cost, relatively large fetal size, multiple fetuses per pregnancy, and short total gestation, this model will allow us to study the mechanism of injury to the exposed spinal cord, and perhaps develop strategies to repair human myelomeningoceles. Copyright (C) 2000 S. Karger AG, Basel.

AB - Objective: Myelomeningocele is a neural tube defect resulting in an exposed spinal cord, which leads to irreversible neurologic damage at birth. We proposed development of a fetal rabbit model of myelomeningocele to study in utero spinal cord injury and repair strategies. Methods: New Zealand white rabbits (n = 10) at 22 days of gestation (term = 31 days) underwent laparotomy to expose the gravid uterus; a hysterotomy exposed the fetal hindlimbs and back. A three to four level lumbar laminectomy was performed, and the dura over the posterior spinal cord was removed. At 30 days of gestation, the does underwent C-section for fetal harvest, and total fetal number, length, weight, and the presence or absence of a spinal defect were recorded for all viable fetuses. Results: All injured fetuses were smaller and weighed less than the nonoperated littermate controls, and histologic examination confirmed a spina bifida-like lesion of their spinal cords. Conclusions: We successfully created an exposed spinal cord defect in the fetal rabbit model similar to the lesion found in humans. Advantageous because of low animal cost, relatively large fetal size, multiple fetuses per pregnancy, and short total gestation, this model will allow us to study the mechanism of injury to the exposed spinal cord, and perhaps develop strategies to repair human myelomeningoceles. Copyright (C) 2000 S. Karger AG, Basel.

KW - Birth defects

KW - Fetal surgery

KW - Myelomeningocele

KW - Neural tube defects

UR - http://www.scopus.com/inward/record.url?scp=0033823168&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033823168&partnerID=8YFLogxK

M3 - Article

C2 - 10971080

AN - SCOPUS:0033823168

VL - 15

SP - 275

EP - 279

JO - Fetal Diagnosis and Therapy

JF - Fetal Diagnosis and Therapy

SN - 1015-3837

IS - 5

ER -