Creation of myelomeningocele in the fetal rabbit

H. Tamiko Housley, Joy L. Graf, Gerald S. Lipshultz, Christopher J. Calvano, Michael R. Harrison, Diana L Farmer, Russel W. Jennings

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Objective: Myelomeningocele is a neural tube defect resulting in an exposed spinal cord, which leads to irreversible neurologic damage at birth. We proposed development of a fetal rabbit model of myelomeningocele to study in utero spinal cord injury and repair strategies. Methods: New Zealand white rabbits (n = 10) at 22 days of gestation (term = 31 days) underwent laparotomy to expose the gravid uterus; a hysterotomy exposed the fetal hindlimbs and back. A three to four level lumbar laminectomy was performed, and the dura over the posterior spinal cord was removed. At 30 days of gestation, the does underwent C-section for fetal harvest, and total fetal number, length, weight, and the presence or absence of a spinal defect were recorded for all viable fetuses. Results: All injured fetuses were smaller and weighed less than the nonoperated littermate controls, and histologic examination confirmed a spina bifida-like lesion of their spinal cords. Conclusions: We successfully created an exposed spinal cord defect in the fetal rabbit model similar to the lesion found in humans. Advantageous because of low animal cost, relatively large fetal size, multiple fetuses per pregnancy, and short total gestation, this model will allow us to study the mechanism of injury to the exposed spinal cord, and perhaps develop strategies to repair human myelomeningoceles. Copyright (C) 2000 S. Karger AG, Basel.

Original languageEnglish (US)
Pages (from-to)275-279
Number of pages5
JournalFetal Diagnosis and Therapy
Volume15
Issue number5
StatePublished - Sep 2000

Keywords

  • Birth defects
  • Fetal surgery
  • Myelomeningocele
  • Neural tube defects

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Pediatrics, Perinatology, and Child Health

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  • Cite this

    Housley, H. T., Graf, J. L., Lipshultz, G. S., Calvano, C. J., Harrison, M. R., Farmer, D. L., & Jennings, R. W. (2000). Creation of myelomeningocele in the fetal rabbit. Fetal Diagnosis and Therapy, 15(5), 275-279.