Creation of a chimeric oncogene: Analysis of the biochemical and biological properties of a v-erbB/src fusion polypeptide

M. L. Privalsky

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

A novel gene was created that linked complementary portions of two different tyrosine kinase oncogenes: v-erbB and v-src. The v-erbB/src chimera encoded a glycoprotein exhibiting the subcellular distribution of the v-erbB protein but containing the kinase catalytic domain of the v-src parent. Fibroblasts expressing the v-erbB/src gene product became transformed to an oncogenic state and closely resembled cells expressing the v-erbB parent oncogene. Our results indicated that v-erbB sequences can be functionally replaced by sequences derived from a different oncogene, v-src, and that important determinants of the transformed phenotype appear to be encoded in oncogene sequences distinct from those defining the kinase catalytic domain itself.

Original languageEnglish (US)
Pages (from-to)1938-1948
Number of pages11
JournalJournal of Virology
Volume61
Issue number6
StatePublished - 1987

ASJC Scopus subject areas

  • Immunology

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