Abstract
A novel gene was created that linked complementary portions of two different tyrosine kinase oncogenes: v-erbB and v-src. The v-erbB/src chimera encoded a glycoprotein exhibiting the subcellular distribution of the v-erbB protein but containing the kinase catalytic domain of the v-src parent. Fibroblasts expressing the v-erbB/src gene product became transformed to an oncogenic state and closely resembled cells expressing the v-erbB parent oncogene. Our results indicated that v-erbB sequences can be functionally replaced by sequences derived from a different oncogene, v-src, and that important determinants of the transformed phenotype appear to be encoded in oncogene sequences distinct from those defining the kinase catalytic domain itself.
Original language | English (US) |
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Pages (from-to) | 1938-1948 |
Number of pages | 11 |
Journal | Journal of Virology |
Volume | 61 |
Issue number | 6 |
State | Published - 1987 |
ASJC Scopus subject areas
- Immunology