Creatinine measurements in 24 h urine by liquid chromatography-tandem mass spectrometry

Eun Kee Park; Takaho Watanabe; Shirley J. Gee; Marc B Schenker; Bruce D. Hammock

doi:10.1021/jf072433s

Creatinine measurements in 24 h urine by liquid chromatography-tandem mass spectrometry

Eun Kee Park, Takaho Watanabe, Shirley J. Gee, Marc B Schenker, Bruce D. Hammock

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

A simple, sensitive, and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determining urinary creatinine was developed and used to evaluate 24 h urine samples collected during an exposure study. Urine (1 μL) was diluted with methanol and then directly applied to LC-MS/MS. Under electrospray ionization (ESI) conditions, the transition molecules of creatinine and creatinine-d₃ were observed at m/z 114 > 44 and m/z 117 > 47, respectively. The retention time of creatinine was 0.59 min. The linear range was 1-2000 ng/mL, with a detection limit in urine of 1 ng/mL. LC-MS/MS and colorimetric end-point methods were significantly associated (R² = 0.8785, p < 0.0001). The LC-MS/MS method to determine creatinine in 24 h urine samples had shorter retention times, was more sensitive, reliable, reproducible, simple, selective, and used a smaller sample size than other LC-MS/MS or commercial methods.

Original language	English (US)
Pages (from-to)	333-336
Number of pages	4
Journal	Journal of Agricultural and Food Chemistry
Volume	56
Issue number	2
DOIs	https://doi.org/10.1021/jf072433s
State	Published - Jan 23 2008

Keywords

Creatinine
LC-MS/MS
Urine
Validation

ASJC Scopus subject areas

Agricultural and Biological Sciences (miscellaneous)
Food Science
Chemistry (miscellaneous)

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10.1021/jf072433s

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title = "Creatinine measurements in 24 h urine by liquid chromatography-tandem mass spectrometry",

abstract = "A simple, sensitive, and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determining urinary creatinine was developed and used to evaluate 24 h urine samples collected during an exposure study. Urine (1 μL) was diluted with methanol and then directly applied to LC-MS/MS. Under electrospray ionization (ESI) conditions, the transition molecules of creatinine and creatinine-d3 were observed at m/z 114 > 44 and m/z 117 > 47, respectively. The retention time of creatinine was 0.59 min. The linear range was 1-2000 ng/mL, with a detection limit in urine of 1 ng/mL. LC-MS/MS and colorimetric end-point methods were significantly associated (R2 = 0.8785, p < 0.0001). The LC-MS/MS method to determine creatinine in 24 h urine samples had shorter retention times, was more sensitive, reliable, reproducible, simple, selective, and used a smaller sample size than other LC-MS/MS or commercial methods.",

keywords = "Creatinine, LC-MS/MS, Urine, Validation",

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AU - Park, Eun Kee

AU - Watanabe, Takaho

AU - Gee, Shirley J.

AU - Schenker, Marc B

AU - Hammock, Bruce D.

PY - 2008/1/23

Y1 - 2008/1/23

N2 - A simple, sensitive, and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determining urinary creatinine was developed and used to evaluate 24 h urine samples collected during an exposure study. Urine (1 μL) was diluted with methanol and then directly applied to LC-MS/MS. Under electrospray ionization (ESI) conditions, the transition molecules of creatinine and creatinine-d3 were observed at m/z 114 > 44 and m/z 117 > 47, respectively. The retention time of creatinine was 0.59 min. The linear range was 1-2000 ng/mL, with a detection limit in urine of 1 ng/mL. LC-MS/MS and colorimetric end-point methods were significantly associated (R2 = 0.8785, p < 0.0001). The LC-MS/MS method to determine creatinine in 24 h urine samples had shorter retention times, was more sensitive, reliable, reproducible, simple, selective, and used a smaller sample size than other LC-MS/MS or commercial methods.

AB - A simple, sensitive, and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determining urinary creatinine was developed and used to evaluate 24 h urine samples collected during an exposure study. Urine (1 μL) was diluted with methanol and then directly applied to LC-MS/MS. Under electrospray ionization (ESI) conditions, the transition molecules of creatinine and creatinine-d3 were observed at m/z 114 > 44 and m/z 117 > 47, respectively. The retention time of creatinine was 0.59 min. The linear range was 1-2000 ng/mL, with a detection limit in urine of 1 ng/mL. LC-MS/MS and colorimetric end-point methods were significantly associated (R2 = 0.8785, p < 0.0001). The LC-MS/MS method to determine creatinine in 24 h urine samples had shorter retention times, was more sensitive, reliable, reproducible, simple, selective, and used a smaller sample size than other LC-MS/MS or commercial methods.

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