Creating cellular micropatterns by switching fouling properties of electroactive ito surfaces

S. S. Shah, J. Y. Lee, A. Revzin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes a novel approach of forming micropatterned co-cultures using a combination of oxygen plasma ashing and electrochemical removal of poly(ethylene glycol) (PEG) silane from indium tin oxide (ITO) substrates. After assembling a layer of PEG silane on ITO, photolithography and oxygen plasma cleaning were used to form cell-adhesive domains within the non-fouling PEG silane layer. After patterning hepatocytes in these domains, the surrounding PEG silane layer was desorbed by applying negative voltage (-1.4 V vs. Ag/AgCl) to the underlying ITO substrate. This switched the fouling properties of the ITO substrate and allowed the adsorption of another cell type creating a micropatterned co-culture.

Original languageEnglish (US)
Title of host publication12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
PublisherChemical and Biological Microsystems Society
Pages257-259
Number of pages3
Publication statusPublished - 2008
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: Oct 12 2008Oct 16 2008

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period10/12/0810/16/08

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Keywords

  • Micropatterned co-culture
  • Photolithography
  • Switchable biointerface

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Shah, S. S., Lee, J. Y., & Revzin, A. (2008). Creating cellular micropatterns by switching fouling properties of electroactive ito surfaces. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference (pp. 257-259). Chemical and Biological Microsystems Society.