Creatine kinase: purification, characterization, and tissue distribution of the MM subtypes

Judith K. Mickelson; C. Jeffrey Carlson; George Kaysen; Elliot Rapaport

doi:10.1016/0009-8981(85)90350-X

Creatine kinase: purification, characterization, and tissue distribution of the MM subtypes

Judith K. Mickelson, C. Jeffrey Carlson, George Kaysen, Elliot Rapaport

Nephrology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The MM isozyme of creatine kinase can be separated into four subtypes by isoelectric focusing: MM₃ (pI 6.90), MM₂ (pI 6.70), MM₁ (pI 6.45), and MM_x (pI 6.25). Individual subtypes were produced and purified from canine heart and then separated by chromatofocusing. Subtypes could not be distinguished by relative molecular mass or kinetic properties. They differed slightly in amino acid composition. Canine and human necropsy tissues were studied to determine subtype distribution. Heart and skeletal muscle contained the most MM isozyme of creatine kinase and it was predominantly of the MM₃ subtype.

Original language	English (US)
Pages (from-to)	181-190
Number of pages	10
Journal	Clinica Chimica Acta
Volume	153
Issue number	3
DOIs	https://doi.org/10.1016/0009-8981(85)90350-X
State	Published - Dec 31 1985

Keywords

Creatine kinase
Isozymes
Myocardium

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry

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10.1016/0009-8981(85)90350-X

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title = "Creatine kinase: purification, characterization, and tissue distribution of the MM subtypes",

abstract = "The MM isozyme of creatine kinase can be separated into four subtypes by isoelectric focusing: MM3 (pI 6.90), MM2 (pI 6.70), MM1 (pI 6.45), and MMx (pI 6.25). Individual subtypes were produced and purified from canine heart and then separated by chromatofocusing. Subtypes could not be distinguished by relative molecular mass or kinetic properties. They differed slightly in amino acid composition. Canine and human necropsy tissues were studied to determine subtype distribution. Heart and skeletal muscle contained the most MM isozyme of creatine kinase and it was predominantly of the MM3 subtype.",

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T2 - purification, characterization, and tissue distribution of the MM subtypes

AU - Mickelson, Judith K.

AU - Carlson, C. Jeffrey

AU - Kaysen, George

AU - Rapaport, Elliot

PY - 1985/12/31

Y1 - 1985/12/31

N2 - The MM isozyme of creatine kinase can be separated into four subtypes by isoelectric focusing: MM3 (pI 6.90), MM2 (pI 6.70), MM1 (pI 6.45), and MMx (pI 6.25). Individual subtypes were produced and purified from canine heart and then separated by chromatofocusing. Subtypes could not be distinguished by relative molecular mass or kinetic properties. They differed slightly in amino acid composition. Canine and human necropsy tissues were studied to determine subtype distribution. Heart and skeletal muscle contained the most MM isozyme of creatine kinase and it was predominantly of the MM3 subtype.

AB - The MM isozyme of creatine kinase can be separated into four subtypes by isoelectric focusing: MM3 (pI 6.90), MM2 (pI 6.70), MM1 (pI 6.45), and MMx (pI 6.25). Individual subtypes were produced and purified from canine heart and then separated by chromatofocusing. Subtypes could not be distinguished by relative molecular mass or kinetic properties. They differed slightly in amino acid composition. Canine and human necropsy tissues were studied to determine subtype distribution. Heart and skeletal muscle contained the most MM isozyme of creatine kinase and it was predominantly of the MM3 subtype.

KW - Creatine kinase

KW - Isozymes

KW - Myocardium

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JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

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ER -

Creatine kinase: purification, characterization, and tissue distribution of the MM subtypes

Abstract

Keywords

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