Abstract
The MM isozyme of creatine kinase can be separated into four subtypes by isoelectric focusing: MM3 (pI 6.90), MM2 (pI 6.70), MM1 (pI 6.45), and MMx (pI 6.25). Individual subtypes were produced and purified from canine heart and then separated by chromatofocusing. Subtypes could not be distinguished by relative molecular mass or kinetic properties. They differed slightly in amino acid composition. Canine and human necropsy tissues were studied to determine subtype distribution. Heart and skeletal muscle contained the most MM isozyme of creatine kinase and it was predominantly of the MM3 subtype.
Original language | English (US) |
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Pages (from-to) | 181-190 |
Number of pages | 10 |
Journal | Clinica Chimica Acta |
Volume | 153 |
Issue number | 3 |
DOIs | |
State | Published - Dec 31 1985 |
Keywords
- Creatine kinase
- Isozymes
- Myocardium
ASJC Scopus subject areas
- Biochemistry
- Clinical Biochemistry