Cranio-lenticulo-sutural dysplasia is caused by a SEC23A mutation leading to abnormal endoplasmic-reticulum-to-Golgi trafficking

Simeon Boyd, J. Christopher Fromme, Jin Ben, Samuel S. Chong, Christopher Nauta, David J. Hur, George Zhang, Susan Hamamoto, Randy Schekman, Mariella Ravazzola, Lelio Orci, Wafaa Eyaid

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Abstract

Cranio-lenticulo-sutural dysplasia (CLSD) is an autosomal recessive syndrome characterized by late-closing fontanels, sutural cataracts, facial dysmorphisms and skeletal defects mapped to chromosome 14q13-q21 (ref. 1). Here we show, using a positional cloning approach, that an F382L amino acid substitution in SEC23A segregates with this syndrome. SEC23A is an essential component of the COPII-coated vesicles that transport secretory proteins from the endoplasmic reticulum to the Golgi complex. Electron microscopy and immunofluorescence show that there is gross dilatation of the endoplasmic reticulum in fibroblasts from individuals affected with CLSD. These cells also exhibit cytoplasmic mislocalization of SEC31. Cell-free vesicle budding assays show that the F382L substitution results in loss of SEC23A function. A phenotype reminiscent of CLSD is observed in zebrafish embryos injected with sec23a-blocking morpholinos. Our observations suggest that disrupted endoplasmic reticulum export of the secretory proteins required for normal morphogenesis accounts for CLSD.

Original languageEnglish (US)
Pages (from-to)1192-1197
Number of pages6
JournalNature Genetics
Volume38
Issue number10
DOIs
StatePublished - Oct 2006

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Endoplasmic Reticulum
Mutation
COP-Coated Vesicles
Morpholinos
Golgi Apparatus
Zebrafish
Amino Acid Substitution
Morphogenesis
Cataract
Fluorescent Antibody Technique
Organism Cloning
Dilatation
Electron Microscopy
Carrier Proteins
Embryonic Structures
Fibroblasts
Chromosomes
Phenotype
Proteins

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Cranio-lenticulo-sutural dysplasia is caused by a SEC23A mutation leading to abnormal endoplasmic-reticulum-to-Golgi trafficking. / Boyd, Simeon; Fromme, J. Christopher; Ben, Jin; Chong, Samuel S.; Nauta, Christopher; Hur, David J.; Zhang, George; Hamamoto, Susan; Schekman, Randy; Ravazzola, Mariella; Orci, Lelio; Eyaid, Wafaa.

In: Nature Genetics, Vol. 38, No. 10, 10.2006, p. 1192-1197.

Research output: Contribution to journalArticle

Boyd, S, Fromme, JC, Ben, J, Chong, SS, Nauta, C, Hur, DJ, Zhang, G, Hamamoto, S, Schekman, R, Ravazzola, M, Orci, L & Eyaid, W 2006, 'Cranio-lenticulo-sutural dysplasia is caused by a SEC23A mutation leading to abnormal endoplasmic-reticulum-to-Golgi trafficking', Nature Genetics, vol. 38, no. 10, pp. 1192-1197. https://doi.org/10.1038/ng1876
Boyd, Simeon ; Fromme, J. Christopher ; Ben, Jin ; Chong, Samuel S. ; Nauta, Christopher ; Hur, David J. ; Zhang, George ; Hamamoto, Susan ; Schekman, Randy ; Ravazzola, Mariella ; Orci, Lelio ; Eyaid, Wafaa. / Cranio-lenticulo-sutural dysplasia is caused by a SEC23A mutation leading to abnormal endoplasmic-reticulum-to-Golgi trafficking. In: Nature Genetics. 2006 ; Vol. 38, No. 10. pp. 1192-1197.
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