Coupling of killer virus transcription with translation in yeast cell-free extracts

F. P. Barbone, Michael J Leibowitz

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The cytoplasmically inherited killer virus of Saccharomyces cerevisiae expresses its dsRNA genome via apparently uncapped viral transcripts produced in the cytoplasm of infected cells. Virions of this naturally temperature-sensitive virus can be added to cell-free translational extracts of uninfected yeast cells resulting in a reaction in which viral transcription and translation are coupled at 15°C in vitro. In this reaction nucleotides are incorporated into full-length transcripts of the M and L-A dsRNA segments, with lower levels of incorporation into genomic RNA. In addition, incorporation of nucleotides is observed into a smaller RNA species showing no sequence relatedness to M or L-A.

Original languageEnglish (US)
Pages (from-to)1755-1760
Number of pages6
JournalJournal of General Virology
Volume72
Issue number7
StatePublished - 1991
Externally publishedYes

Fingerprint

Cell Extracts
Yeasts
Viruses
Nucleotides
RNA
Virion
Saccharomyces cerevisiae
Cytoplasm
Genome
Temperature

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Coupling of killer virus transcription with translation in yeast cell-free extracts. / Barbone, F. P.; Leibowitz, Michael J.

In: Journal of General Virology, Vol. 72, No. 7, 1991, p. 1755-1760.

Research output: Contribution to journalArticle

@article{ccd146200b2d4e55ad4c66028c12c68e,
title = "Coupling of killer virus transcription with translation in yeast cell-free extracts",
abstract = "The cytoplasmically inherited killer virus of Saccharomyces cerevisiae expresses its dsRNA genome via apparently uncapped viral transcripts produced in the cytoplasm of infected cells. Virions of this naturally temperature-sensitive virus can be added to cell-free translational extracts of uninfected yeast cells resulting in a reaction in which viral transcription and translation are coupled at 15°C in vitro. In this reaction nucleotides are incorporated into full-length transcripts of the M and L-A dsRNA segments, with lower levels of incorporation into genomic RNA. In addition, incorporation of nucleotides is observed into a smaller RNA species showing no sequence relatedness to M or L-A.",
author = "Barbone, {F. P.} and Leibowitz, {Michael J}",
year = "1991",
language = "English (US)",
volume = "72",
pages = "1755--1760",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "7",

}

TY - JOUR

T1 - Coupling of killer virus transcription with translation in yeast cell-free extracts

AU - Barbone, F. P.

AU - Leibowitz, Michael J

PY - 1991

Y1 - 1991

N2 - The cytoplasmically inherited killer virus of Saccharomyces cerevisiae expresses its dsRNA genome via apparently uncapped viral transcripts produced in the cytoplasm of infected cells. Virions of this naturally temperature-sensitive virus can be added to cell-free translational extracts of uninfected yeast cells resulting in a reaction in which viral transcription and translation are coupled at 15°C in vitro. In this reaction nucleotides are incorporated into full-length transcripts of the M and L-A dsRNA segments, with lower levels of incorporation into genomic RNA. In addition, incorporation of nucleotides is observed into a smaller RNA species showing no sequence relatedness to M or L-A.

AB - The cytoplasmically inherited killer virus of Saccharomyces cerevisiae expresses its dsRNA genome via apparently uncapped viral transcripts produced in the cytoplasm of infected cells. Virions of this naturally temperature-sensitive virus can be added to cell-free translational extracts of uninfected yeast cells resulting in a reaction in which viral transcription and translation are coupled at 15°C in vitro. In this reaction nucleotides are incorporated into full-length transcripts of the M and L-A dsRNA segments, with lower levels of incorporation into genomic RNA. In addition, incorporation of nucleotides is observed into a smaller RNA species showing no sequence relatedness to M or L-A.

UR - http://www.scopus.com/inward/record.url?scp=0025768267&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025768267&partnerID=8YFLogxK

M3 - Article

C2 - 1713263

AN - SCOPUS:0025768267

VL - 72

SP - 1755

EP - 1760

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 7

ER -