Coupling between microtubule sliding, plus-end growth and spindle length revealed by kinesin-8 depletion

Haifeng Wang, Ingrid Brust-Mascher, Dhanya Cheerambathur, Jonathan M. Scholey

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Mitotic spindle length control requires coordination between microtubule (MT) dynamics and motor-generated forces. To investigate how MT plus-end polymerization contributes to spindle length in Drosophila embryos, we studied the dynamics of the MT plus-end depolymerase, kinesin-8, and the effects of kinesin-8 inhibition using mutants and antibody microinjection. As expected, kinesin-8 was found to contribute to anaphase A. Furthermore, kinesin-8 depletion caused: (i) excessive polymerization of interpolar (ip) MT plus ends, which "overgrow" to penetrate distal half spindles; (ii) an increase in the poleward ipMT sliding rate that is coupled to MT plus-end polymerization; (iii) premature spindle elongation during metaphase/anaphase A; and (iv) an increase in the anaphase B spindle elongation rate which correlates linearly with the MT sliding rate. This is best explained by a revised "ipMT sliding/minus-end depolymerization" model for spindle length control which incorporates a coupling between ipMT plus end dynamics and the outward ipMT sliding that drives poleward flux and spindle elongation.

Original languageEnglish (US)
Pages (from-to)715-728
Number of pages14
JournalCytoskeleton
Volume67
Issue number11
DOIs
StatePublished - Nov 2010

Keywords

  • KLP67A
  • Microtubule dynamics
  • Mitosis
  • Poleward flux
  • Spindle length control

ASJC Scopus subject areas

  • Cell Biology
  • Structural Biology

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