Counting and breaking individual biological bonds

Force spectroscopy of tethered ligand-receptor pairs

Raymond W. Friddle, Todd A. Sulchek, Huguette Albrecht, Sally J. De Nardo, Aleksandr Noy

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Force spectroscopy provides a direct approach for probing biological interactions at the single-molecule level. Tethered sytems, in which flexible polymer linkers connect the interacting molecules to the surfaces of the atomic force microscope probe and sample, provide a particularly attractive platform for studying such interactions. We will review the basic physical principles of force spectroscopy measurements in these systems, and show that mechanical properties of the tether linkages allow independent determination of the bond rupture forces and the number of ruptured bonds. Forces measured in these systems obey the predictions of a Markovian model for the strength of multiple parallel bonds. Finally, we discuss the use of the dynamic force pectra of single and multiple protein-ligand bonds for determination of kinetic parameters for multivalent interactions.

Original languageEnglish (US)
Pages (from-to)41-48
Number of pages8
JournalCurrent Nanoscience
Volume3
Issue number1
DOIs
StatePublished - Feb 2007
Externally publishedYes

Fingerprint

Spectrum Analysis
Ligands
Spectroscopy
Molecules
Kinetic parameters
Rupture
Polymers
Microscopes
Proteins
Mechanical properties

Keywords

  • Atomic force microscope (AFM)
  • Entropic elasticity
  • Freely Jointed Chain (FJC)
  • Mucin 1 (MUC1)
  • PEG

ASJC Scopus subject areas

  • Biotechnology
  • Pharmaceutical Science

Cite this

Counting and breaking individual biological bonds : Force spectroscopy of tethered ligand-receptor pairs. / Friddle, Raymond W.; Sulchek, Todd A.; Albrecht, Huguette; De Nardo, Sally J.; Noy, Aleksandr.

In: Current Nanoscience, Vol. 3, No. 1, 02.2007, p. 41-48.

Research output: Contribution to journalArticle

Friddle, Raymond W. ; Sulchek, Todd A. ; Albrecht, Huguette ; De Nardo, Sally J. ; Noy, Aleksandr. / Counting and breaking individual biological bonds : Force spectroscopy of tethered ligand-receptor pairs. In: Current Nanoscience. 2007 ; Vol. 3, No. 1. pp. 41-48.
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