Cotranscriptional set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex

Michael Christopher Keogh; Siavash K. Kurdistani; Stephanie A. Morris; Seong Hoon Ahn; Vladimir Podolny; Sean R. Collins; Maya Schuldiner; Kayu Chin; Thanuja Punna; Natalie J. Thompson; Charles Boone; Andrew Emili; Jonathan S. Weissman; Timothy R. Hughes; Brian D. Strahl; Michael Grunstein; Jack F. Greenblatt; Stephen Buratowski; Nevan J. Krogan

doi:10.1016/j.cell.2005.10.025

Cotranscriptional set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex

Michael Christopher Keogh, Siavash K. Kurdistani, Stephanie A. Morris, Seong Hoon Ahn, Vladimir Podolny, Sean R. Collins, Maya Schuldiner, Kayu Chin, Thanuja Punna, Natalie J. Thompson, Charles Boone, Andrew Emili, Jonathan S. Weissman, Timothy R. Hughes, Brian D. Strahl, Michael Grunstein, Jack F. Greenblatt, Stephen Buratowski, Nevan J. Krogan

Microbiology and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

572 Scopus citations

Abstract

The yeast histone deacetylase Rpd3 can be recruited to promoters to repress transcription initiation. Biochemical, genetic, and gene-expression analyses show that Rpd3 exists in two distinct complexes. The smaller complex, Rpd3C(S), shares Sin3 and Ume1 with Rpd3C(L) but contains the unique subunits Rco1 and Eaf3. Rpd3C(S) mutants exhibit phenotypes remarkably similar to those of Set2, a histone methyltransferase associated with elongating RNA polymerase II. Chromatin immunoprecipitation and biochemical experiments indicate that the chromodomain of Eaf3 recruits Rpd3C(S) to nucleosomes methylated by Set2 on histone H3 lysine 36, leading to deacetylation of transcribed regions. This pathway apparently acts to negatively regulate transcription because deleting the genes for Set2 or Rpd3C(S) bypasses the requirement for the positive elongation factor Bur1/Bur2.

Original language	English (US)
Pages (from-to)	593-605
Number of pages	13
Journal	Cell
Volume	123
Issue number	4
DOIs	https://doi.org/10.1016/j.cell.2005.10.025
State	Published - Nov 18 2005

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/j.cell.2005.10.025

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Keogh, M. C., Kurdistani, S. K., Morris, S. A., Ahn, S. H., Podolny, V., Collins, S. R., Schuldiner, M., Chin, K., Punna, T., Thompson, N. J., Boone, C., Emili, A., Weissman, J. S., Hughes, T. R., Strahl, B. D., Grunstein, M., Greenblatt, J. F., Buratowski, S., & Krogan, N. J. (2005). Cotranscriptional set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex. Cell, 123(4), 593-605. https://doi.org/10.1016/j.cell.2005.10.025

Cotranscriptional set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex. / Keogh, Michael Christopher; Kurdistani, Siavash K.; Morris, Stephanie A.; Ahn, Seong Hoon; Podolny, Vladimir; Collins, Sean R.; Schuldiner, Maya; Chin, Kayu; Punna, Thanuja; Thompson, Natalie J.; Boone, Charles; Emili, Andrew; Weissman, Jonathan S.; Hughes, Timothy R.; Strahl, Brian D.; Grunstein, Michael; Greenblatt, Jack F.; Buratowski, Stephen; Krogan, Nevan J.

In: Cell, Vol. 123, No. 4, 18.11.2005, p. 593-605.

Research output: Contribution to journal › Article › peer-review

Keogh, MC, Kurdistani, SK, Morris, SA, Ahn, SH, Podolny, V, Collins, SR, Schuldiner, M, Chin, K, Punna, T, Thompson, NJ, Boone, C, Emili, A, Weissman, JS, Hughes, TR, Strahl, BD, Grunstein, M, Greenblatt, JF, Buratowski, S & Krogan, NJ 2005, 'Cotranscriptional set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex', Cell, vol. 123, no. 4, pp. 593-605. https://doi.org/10.1016/j.cell.2005.10.025

Keogh, Michael Christopher ; Kurdistani, Siavash K. ; Morris, Stephanie A. ; Ahn, Seong Hoon ; Podolny, Vladimir ; Collins, Sean R. ; Schuldiner, Maya ; Chin, Kayu ; Punna, Thanuja ; Thompson, Natalie J. ; Boone, Charles ; Emili, Andrew ; Weissman, Jonathan S. ; Hughes, Timothy R. ; Strahl, Brian D. ; Grunstein, Michael ; Greenblatt, Jack F. ; Buratowski, Stephen ; Krogan, Nevan J. / Cotranscriptional set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex. In: Cell. 2005 ; Vol. 123, No. 4. pp. 593-605.

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abstract = "The yeast histone deacetylase Rpd3 can be recruited to promoters to repress transcription initiation. Biochemical, genetic, and gene-expression analyses show that Rpd3 exists in two distinct complexes. The smaller complex, Rpd3C(S), shares Sin3 and Ume1 with Rpd3C(L) but contains the unique subunits Rco1 and Eaf3. Rpd3C(S) mutants exhibit phenotypes remarkably similar to those of Set2, a histone methyltransferase associated with elongating RNA polymerase II. Chromatin immunoprecipitation and biochemical experiments indicate that the chromodomain of Eaf3 recruits Rpd3C(S) to nucleosomes methylated by Set2 on histone H3 lysine 36, leading to deacetylation of transcribed regions. This pathway apparently acts to negatively regulate transcription because deleting the genes for Set2 or Rpd3C(S) bypasses the requirement for the positive elongation factor Bur1/Bur2.",

author = "Keogh, {Michael Christopher} and Kurdistani, {Siavash K.} and Morris, {Stephanie A.} and Ahn, {Seong Hoon} and Vladimir Podolny and Collins, {Sean R.} and Maya Schuldiner and Kayu Chin and Thanuja Punna and Thompson, {Natalie J.} and Charles Boone and Andrew Emili and Weissman, {Jonathan S.} and Hughes, {Timothy R.} and Strahl, {Brian D.} and Michael Grunstein and Greenblatt, {Jack F.} and Stephen Buratowski and Krogan, {Nevan J.}",

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AU - Keogh, Michael Christopher

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AU - Ahn, Seong Hoon

AU - Podolny, Vladimir

AU - Collins, Sean R.

AU - Schuldiner, Maya

AU - Chin, Kayu

AU - Punna, Thanuja

AU - Thompson, Natalie J.

AU - Boone, Charles

AU - Emili, Andrew

AU - Weissman, Jonathan S.

AU - Hughes, Timothy R.

AU - Strahl, Brian D.

AU - Grunstein, Michael

AU - Greenblatt, Jack F.

AU - Buratowski, Stephen

AU - Krogan, Nevan J.

PY - 2005/11/18

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N2 - The yeast histone deacetylase Rpd3 can be recruited to promoters to repress transcription initiation. Biochemical, genetic, and gene-expression analyses show that Rpd3 exists in two distinct complexes. The smaller complex, Rpd3C(S), shares Sin3 and Ume1 with Rpd3C(L) but contains the unique subunits Rco1 and Eaf3. Rpd3C(S) mutants exhibit phenotypes remarkably similar to those of Set2, a histone methyltransferase associated with elongating RNA polymerase II. Chromatin immunoprecipitation and biochemical experiments indicate that the chromodomain of Eaf3 recruits Rpd3C(S) to nucleosomes methylated by Set2 on histone H3 lysine 36, leading to deacetylation of transcribed regions. This pathway apparently acts to negatively regulate transcription because deleting the genes for Set2 or Rpd3C(S) bypasses the requirement for the positive elongation factor Bur1/Bur2.

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