Corticotropin-releasing factor-binding protein (CRF-BP) inhibits CRF- and urotensin-I-mediated activation of CRF receptor-1 and -2 in common carp

Remy Manuel, Juriaan R. Metz, Gert Flik, Wylie W. Vale, Mark O. Huising

Research output: Contribution to journalArticlepeer-review

Abstract

Corticotropin-releasing factor-binding protein (CRF-BP) is considered a key determinant for CRF receptor (CRF-R) activation by CRF and several related peptides. Earlier studies have shown that the CRF system is highly conserved in gene structures throughout evolution, yet little is known about the evolutionary conservation of its biological functions. Therefore, we address the functional properties of CRF-BP and CRF-Rs in a teleost fish (common carp; Cyprinus carpio L.). We report the finding of two similar, yet distinct, genes for both CRF-R1 and CRF-R2 in this species. The four receptors are differentially responsive to CRF, urotensin-I (UI), sauvagine, and urocortin-2 (Ucn-2) and -3 (Ucn-3) as shown by luciferase assays. In vitro, carp CRF-BP inhibits CRF- and UI-mediated activation of the newfound CRF-Rs, but its potency to do so varies between receptor and peptide ligand. This is the first paper to establish the functionality and physiological interplay between CRF-BP, CRF-Rs and CRF-family peptides in a teleostean species.

Original languageEnglish (US)
Pages (from-to)69-75
Number of pages7
JournalGeneral and Comparative Endocrinology
Volume202
DOIs
StatePublished - Jun 1 2014
Externally publishedYes

Keywords

  • Common carp
  • CRF
  • CRF-BP
  • CRF-R

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology

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