Abstract
We hypothesize that real-time in vivo microvascular abnormalities should correlate with biochemical markers of inflammation/endothelial dysfunction in T1DM. Real-time quantification of T1DM and healthy non-diabetic control microcirculation was conducted utilizing computer-assisted intravital microscopy. Selected biochemical markers (high sensitivity C-reactive protein (hsCRP), soluble vascular cell adhesion molecules (sVCAM), soluble intercellular adhesion molecules (sICAM), soluble E-selectin (sE-selectin), nitrotyrosine, superoxide anion (O2 -), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α)) were used for correlation. The severity of microvascular abnormalities, as reflected by the arithmetic severity index (SI), was significantly increased in T1DM vs. controls (5.89 ± 1.47 vs. 2.34 ± 1.48; P<0.001). In addition several of the specific microvascular abnormalities (related to flow and morphometry) were significantly more prevalent in the T1DM patients. Finally, the following significant positive correlations existed between the inflammatory/endothelial dysfunction markers and specific microvascular abnormalities: sVCAM and abnormal vessel diameter (P=0.004, OR =1.033, 95% CI for OR =(1.01, 1.056)), superoxide (O 2 -) release and abnormal vessel distribution (P=0.032, OR =1.798, 95% CI for OR =(1.051, 3.075)), and sE-selectin and abnormal vessel distribution (P=0.036, OR =1.118, 95% CI for OR =(1.007, 1.241)). In view of such significant correlations, we conclude that these specific microvascular abnormalities can serve as unique physiologic markers of endothelial dysfunction to correlate with the biochemical markers of inflammatory/endothelial dysfunction in disease progression and therapeutic efficacy studies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 285-295 |
| Number of pages | 11 |
| Journal | Clinical Hemorheology and Microcirculation |
| Volume | 42 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2009 |
Fingerprint
Keywords
- Biochemical markers
- Computer-assisted intravital microscopy
- Microvascular abnormalities
- Physiologic markers
- T1DM
ASJC Scopus subject areas
- Hematology
- Physiology
- Physiology (medical)
- Cardiology and Cardiovascular Medicine
Cite this
Correlation of microvascular abnormalities and endothelial dysfunction in Type-1 diabetes mellitus (T1DM) : A real-time intravital microscopy study. / Cheung, Anthony T W; Tomic, M. Meighan; Chen, Peter C Y; Miguelino, Eric; Li, Chin-Shang; Devaraj, Sridevi.
In: Clinical Hemorheology and Microcirculation, Vol. 42, No. 4, 2009, p. 285-295.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Correlation of microvascular abnormalities and endothelial dysfunction in Type-1 diabetes mellitus (T1DM)
T2 - A real-time intravital microscopy study
AU - Cheung, Anthony T W
AU - Tomic, M. Meighan
AU - Chen, Peter C Y
AU - Miguelino, Eric
AU - Li, Chin-Shang
AU - Devaraj, Sridevi
PY - 2009
Y1 - 2009
N2 - We hypothesize that real-time in vivo microvascular abnormalities should correlate with biochemical markers of inflammation/endothelial dysfunction in T1DM. Real-time quantification of T1DM and healthy non-diabetic control microcirculation was conducted utilizing computer-assisted intravital microscopy. Selected biochemical markers (high sensitivity C-reactive protein (hsCRP), soluble vascular cell adhesion molecules (sVCAM), soluble intercellular adhesion molecules (sICAM), soluble E-selectin (sE-selectin), nitrotyrosine, superoxide anion (O2 -), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α)) were used for correlation. The severity of microvascular abnormalities, as reflected by the arithmetic severity index (SI), was significantly increased in T1DM vs. controls (5.89 ± 1.47 vs. 2.34 ± 1.48; P<0.001). In addition several of the specific microvascular abnormalities (related to flow and morphometry) were significantly more prevalent in the T1DM patients. Finally, the following significant positive correlations existed between the inflammatory/endothelial dysfunction markers and specific microvascular abnormalities: sVCAM and abnormal vessel diameter (P=0.004, OR =1.033, 95% CI for OR =(1.01, 1.056)), superoxide (O 2 -) release and abnormal vessel distribution (P=0.032, OR =1.798, 95% CI for OR =(1.051, 3.075)), and sE-selectin and abnormal vessel distribution (P=0.036, OR =1.118, 95% CI for OR =(1.007, 1.241)). In view of such significant correlations, we conclude that these specific microvascular abnormalities can serve as unique physiologic markers of endothelial dysfunction to correlate with the biochemical markers of inflammatory/endothelial dysfunction in disease progression and therapeutic efficacy studies.
AB - We hypothesize that real-time in vivo microvascular abnormalities should correlate with biochemical markers of inflammation/endothelial dysfunction in T1DM. Real-time quantification of T1DM and healthy non-diabetic control microcirculation was conducted utilizing computer-assisted intravital microscopy. Selected biochemical markers (high sensitivity C-reactive protein (hsCRP), soluble vascular cell adhesion molecules (sVCAM), soluble intercellular adhesion molecules (sICAM), soluble E-selectin (sE-selectin), nitrotyrosine, superoxide anion (O2 -), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α)) were used for correlation. The severity of microvascular abnormalities, as reflected by the arithmetic severity index (SI), was significantly increased in T1DM vs. controls (5.89 ± 1.47 vs. 2.34 ± 1.48; P<0.001). In addition several of the specific microvascular abnormalities (related to flow and morphometry) were significantly more prevalent in the T1DM patients. Finally, the following significant positive correlations existed between the inflammatory/endothelial dysfunction markers and specific microvascular abnormalities: sVCAM and abnormal vessel diameter (P=0.004, OR =1.033, 95% CI for OR =(1.01, 1.056)), superoxide (O 2 -) release and abnormal vessel distribution (P=0.032, OR =1.798, 95% CI for OR =(1.051, 3.075)), and sE-selectin and abnormal vessel distribution (P=0.036, OR =1.118, 95% CI for OR =(1.007, 1.241)). In view of such significant correlations, we conclude that these specific microvascular abnormalities can serve as unique physiologic markers of endothelial dysfunction to correlate with the biochemical markers of inflammatory/endothelial dysfunction in disease progression and therapeutic efficacy studies.
KW - Biochemical markers
KW - Computer-assisted intravital microscopy
KW - Microvascular abnormalities
KW - Physiologic markers
KW - T1DM
UR - http://www.scopus.com/inward/record.url?scp=68649107103&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=68649107103&partnerID=8YFLogxK
U2 - 10.3233/CH-2009-1199
DO - 10.3233/CH-2009-1199
M3 - Article
C2 - 19628894
AN - SCOPUS:68649107103
VL - 42
SP - 285
EP - 295
JO - Clinical Hemorheology and Microcirculation
JF - Clinical Hemorheology and Microcirculation
SN - 1386-0291
IS - 4
ER -