Correlation of microvascular abnormalities and endothelial dysfunction in Type-1 diabetes mellitus (T1DM): A real-time intravital microscopy study

Anthony T W Cheung, M. Meighan Tomic, Peter C Y Chen, Eric Miguelino, Chin-Shang Li, Sridevi Devaraj

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We hypothesize that real-time in vivo microvascular abnormalities should correlate with biochemical markers of inflammation/endothelial dysfunction in T1DM. Real-time quantification of T1DM and healthy non-diabetic control microcirculation was conducted utilizing computer-assisted intravital microscopy. Selected biochemical markers (high sensitivity C-reactive protein (hsCRP), soluble vascular cell adhesion molecules (sVCAM), soluble intercellular adhesion molecules (sICAM), soluble E-selectin (sE-selectin), nitrotyrosine, superoxide anion (O2 -), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α)) were used for correlation. The severity of microvascular abnormalities, as reflected by the arithmetic severity index (SI), was significantly increased in T1DM vs. controls (5.89 ± 1.47 vs. 2.34 ± 1.48; P<0.001). In addition several of the specific microvascular abnormalities (related to flow and morphometry) were significantly more prevalent in the T1DM patients. Finally, the following significant positive correlations existed between the inflammatory/endothelial dysfunction markers and specific microvascular abnormalities: sVCAM and abnormal vessel diameter (P=0.004, OR =1.033, 95% CI for OR =(1.01, 1.056)), superoxide (O 2 -) release and abnormal vessel distribution (P=0.032, OR =1.798, 95% CI for OR =(1.051, 3.075)), and sE-selectin and abnormal vessel distribution (P=0.036, OR =1.118, 95% CI for OR =(1.007, 1.241)). In view of such significant correlations, we conclude that these specific microvascular abnormalities can serve as unique physiologic markers of endothelial dysfunction to correlate with the biochemical markers of inflammatory/endothelial dysfunction in disease progression and therapeutic efficacy studies.

Original languageEnglish (US)
Pages (from-to)285-295
Number of pages11
JournalClinical Hemorheology and Microcirculation
Volume42
Issue number4
DOIs
StatePublished - 2009

Fingerprint

Type 1 Diabetes Mellitus
E-Selectin
Vascular Cell Adhesion Molecule-1
Biomarkers
Superoxides
Cell Adhesion Molecules
Microcirculation
Interleukin-1
C-Reactive Protein
Disease Progression
Tumor Necrosis Factor-alpha
Inflammation
Intravital Microscopy
Therapeutics

Keywords

  • Biochemical markers
  • Computer-assisted intravital microscopy
  • Microvascular abnormalities
  • Physiologic markers
  • T1DM

ASJC Scopus subject areas

  • Hematology
  • Physiology
  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

Correlation of microvascular abnormalities and endothelial dysfunction in Type-1 diabetes mellitus (T1DM) : A real-time intravital microscopy study. / Cheung, Anthony T W; Tomic, M. Meighan; Chen, Peter C Y; Miguelino, Eric; Li, Chin-Shang; Devaraj, Sridevi.

In: Clinical Hemorheology and Microcirculation, Vol. 42, No. 4, 2009, p. 285-295.

Research output: Contribution to journalArticle

Cheung, Anthony T W ; Tomic, M. Meighan ; Chen, Peter C Y ; Miguelino, Eric ; Li, Chin-Shang ; Devaraj, Sridevi. / Correlation of microvascular abnormalities and endothelial dysfunction in Type-1 diabetes mellitus (T1DM) : A real-time intravital microscopy study. In: Clinical Hemorheology and Microcirculation. 2009 ; Vol. 42, No. 4. pp. 285-295.
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AB - We hypothesize that real-time in vivo microvascular abnormalities should correlate with biochemical markers of inflammation/endothelial dysfunction in T1DM. Real-time quantification of T1DM and healthy non-diabetic control microcirculation was conducted utilizing computer-assisted intravital microscopy. Selected biochemical markers (high sensitivity C-reactive protein (hsCRP), soluble vascular cell adhesion molecules (sVCAM), soluble intercellular adhesion molecules (sICAM), soluble E-selectin (sE-selectin), nitrotyrosine, superoxide anion (O2 -), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α)) were used for correlation. The severity of microvascular abnormalities, as reflected by the arithmetic severity index (SI), was significantly increased in T1DM vs. controls (5.89 ± 1.47 vs. 2.34 ± 1.48; P<0.001). In addition several of the specific microvascular abnormalities (related to flow and morphometry) were significantly more prevalent in the T1DM patients. Finally, the following significant positive correlations existed between the inflammatory/endothelial dysfunction markers and specific microvascular abnormalities: sVCAM and abnormal vessel diameter (P=0.004, OR =1.033, 95% CI for OR =(1.01, 1.056)), superoxide (O 2 -) release and abnormal vessel distribution (P=0.032, OR =1.798, 95% CI for OR =(1.051, 3.075)), and sE-selectin and abnormal vessel distribution (P=0.036, OR =1.118, 95% CI for OR =(1.007, 1.241)). In view of such significant correlations, we conclude that these specific microvascular abnormalities can serve as unique physiologic markers of endothelial dysfunction to correlate with the biochemical markers of inflammatory/endothelial dysfunction in disease progression and therapeutic efficacy studies.

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