Correlation of Ki-67, p53, and Adnab-9 immunohistochemical staining and ploidy with clinical and histopathologic features of severely dysplastic colorectal adenomas

Rafiq A. Sheikh, Byung Hee Min, Shagufta Yasmeen, Raymond Teplitz, Henry Tesluk, Boris Henry Ruebner, Martin Tobi, James Hatfield, Suzanne Fligiel, Michael J. Lawson

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Variations of Ki-67, p53, and Adnab-9 monoclonal antibody reactions in colonic adenomas may be associated with colonic cancer risk. We studied the predictive value of these markers for adverse behavior in severely dysplastic colorectal adenomas, such as an associated carcinoma, multiplicity of adenomas, and subsequent development of adenomas. For this purpose we compared the clinical, gross, and histologic characteristics of highly dysplastic index polyps in 42 patients with Ki 67, p53, and Adnab-9 immunostaining and other molecular markers. Polyps were removed endoscopically, and severely dysplastic polyps were stained immunohistochemically with Ki-67, Adnab-9, and p53 protein by the avidin biotin conjugate (ABC) technique. Quantitative DNA (QDNA) was analyzed by computer-assisted image analysis. Ki-67 immunohistochemistry showed reversal of normal distribution of nuclear staining from the normal basal position to the upper third of the colonic crypts. This abnormality of immunostaining in dysplastic adenomas was the earliest detected by the panel we used. A statistically significant correlation was seen between invasiveness of carcinoma in the index polyp and polyp size (P = 0.003), sessile morphology (P = 0.037), and villous or tubulovillous histology (P = 0.019). In the index adenoma, p53 positivity was correlated with multiplicity at initial examination (P = 0.053), villous histology (P = 0.053), invasiveness of carcinoma (P < 0.003), and recurrence of colorectal adenomas (P = 0.025). Although p53 positivity and aneuploidy were correlated with invasiveness of carcinoma in the index polyp (P = 0.025), Adnab-9 positivity was not. However, Adnab-9 positivity in the index polyp was associated with multiplicity of adenomas (P = 0.04) as well as recurrence of adenomas (P < 0.024). In conclusion, in addition to the morphologic and histologic markers already known, Ki-67, Adnab-9 antibody, and p53 protein may be prognostic indicators useful in follow-up of patients with severely dysplastic colorectal adenomas. Adnab-9 antibody may identify a field defect in above-average-risk adenoma-bearing patients.

Original languageEnglish (US)
Pages (from-to)223-229
Number of pages7
JournalDigestive Diseases and Sciences
Volume48
Issue number1
DOIs
StatePublished - Jan 1 2003

Keywords

  • Adenoma
  • Colorectal neoplasms
  • Follow-up studies
  • Ki-67
  • Monoclonal antibodies
  • Neoplasm invasiveness
  • P53 genes
  • p53 protein

ASJC Scopus subject areas

  • Gastroenterology

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    Sheikh, R. A., Min, B. H., Yasmeen, S., Teplitz, R., Tesluk, H., Ruebner, B. H., Tobi, M., Hatfield, J., Fligiel, S., & Lawson, M. J. (2003). Correlation of Ki-67, p53, and Adnab-9 immunohistochemical staining and ploidy with clinical and histopathologic features of severely dysplastic colorectal adenomas. Digestive Diseases and Sciences, 48(1), 223-229. https://doi.org/10.1023/A:1021727608133