Correlation of immune activation with HIV-1 RNA levels assayed by real-time RT-PCR in HIV-1 subtype C infected patients in Northern India

Atima Agarwal, Sumathi Sankaran-Walters, Madhu Vajpayee, V. Sreenivas, Pradeep Seth, Satya Dandekar

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Background: Assays with specificity and cost effectiveness are needed for the measurement of HIV-1 burden to monitor disease progression or response to anti-retroviral therapy (ART) in HIV-1 subtype C infected patients. Objectives: The objective of this study was to develop and validate an affordable one step real-time RT-PCR assay with high specificity and sensitivity to measure plasma HIV-1 loads in HIV-1 subtype C infected patients. Results: We developed an RT-PCR assay to detect and quantitate plasma HIV-1 levels in HIV-1 subtype C infected patients. An inverse correlation between plasma viral loads (PVL) and CD4+ T-cell numbers was detected at all CDC stages. Significant correlations were found between CD8+ T-cell activation and PVL, as well as with the clinical and immunological status of the patients. Conclusions: This RT-PCR assay provides a sensitive method to measure PVL in HIV-1 subtype C infected patients. Viral loads correlated with immune activation and can be used to monitor HIV care in India.

Original languageEnglish (US)
Pages (from-to)301-306
Number of pages6
JournalJournal of Clinical Virology
Volume40
Issue number4
DOIs
StatePublished - Dec 2007

Keywords

  • HIV-1 subtype C
  • HIV-1 viral load
  • Real-time RT-PCR

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

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