Correlation of DNA methylation levels in blood and saliva DNA in young girls of the LEGACY girls study

Hui Chen Wu, Qiao Wang, Wendy K. Chung, Irene L. Andrulis, Mary B. Daly, Esther M. John, Theresa H Keegan, Julia Knight, Angela R. Bradbury, Maya A. Kappil, Irina Gurvich, Regina M. Santella, Mary Beth Terry

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Many epidemiologic studies of environmental exposures and disease susceptibility measure DNA methylation in white blood cells (WBC). Some studies are also starting to use saliva DNA as it is usually more readily available in large epidemiologic studies. However, little is known about the correlation of methylation between WBC and saliva DNA. We examined DNA methylation in three repetitive elements, Sat2, Alu, and LINE-1, and in four CpG sites, including AHRR (cg23576855, cg05575921), cg05951221 at 2q37.1, and cg11924019 at CYP1A1, in 57 girls aged 6-15 years with blood and saliva collected on the same day. We measured all DNA methylation markers by bisulfite-pyrosequencing, except for Sat2 and Alu, which were measured by the MethyLight assay. Methylation levels measured in saliva DNA were lower than those in WBC DNA, with differences ranging from 2.8% for Alu to 14.1% for cg05575921. Methylation levels for the three repetitive elements measured in saliva DNA were all positively correlated with those in WBC DNA. However, there was a wide range in the Spearman correlations, with the smallest correlation found for Alu (0.24) and the strongest correlation found for LINE-1 (0.73). Spearman correlations for cg05575921, cg05951221, and cg11924019 were 0.33, 0.42, and 0.79, respectively. If these findings are replicated in larger studies, they suggest that, for selected methylation markers (e.g., LINE-1), methylation levels may be highly correlated between blood and saliva, while for others methylation markers, the levels may be more tissue specific. Thus, in studies that differ by DNA source, each interrogated site should be separately examined in order to evaluate the correlation in DNA methylation levels across DNA sources.

Original languageEnglish (US)
Pages (from-to)929-933
Number of pages5
JournalEpigenetics
Volume9
Issue number7
DOIs
StatePublished - Apr 22 2014
Externally publishedYes

Fingerprint

DNA Methylation
Saliva
Methylation
DNA
Leukocytes
Epidemiologic Studies
Alu Elements
Cytochrome P-450 CYP1A1
Disease Susceptibility
Environmental Exposure
Genetic Markers

Keywords

  • Buccal cells
  • DNA genomic methylation
  • LINE-1
  • Pyrosequencing
  • Saliva
  • White blood cells

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research

Cite this

Wu, H. C., Wang, Q., Chung, W. K., Andrulis, I. L., Daly, M. B., John, E. M., ... Terry, M. B. (2014). Correlation of DNA methylation levels in blood and saliva DNA in young girls of the LEGACY girls study. Epigenetics, 9(7), 929-933. https://doi.org/10.4161/epi.28902

Correlation of DNA methylation levels in blood and saliva DNA in young girls of the LEGACY girls study. / Wu, Hui Chen; Wang, Qiao; Chung, Wendy K.; Andrulis, Irene L.; Daly, Mary B.; John, Esther M.; Keegan, Theresa H; Knight, Julia; Bradbury, Angela R.; Kappil, Maya A.; Gurvich, Irina; Santella, Regina M.; Terry, Mary Beth.

In: Epigenetics, Vol. 9, No. 7, 22.04.2014, p. 929-933.

Research output: Contribution to journalArticle

Wu, HC, Wang, Q, Chung, WK, Andrulis, IL, Daly, MB, John, EM, Keegan, TH, Knight, J, Bradbury, AR, Kappil, MA, Gurvich, I, Santella, RM & Terry, MB 2014, 'Correlation of DNA methylation levels in blood and saliva DNA in young girls of the LEGACY girls study', Epigenetics, vol. 9, no. 7, pp. 929-933. https://doi.org/10.4161/epi.28902
Wu, Hui Chen ; Wang, Qiao ; Chung, Wendy K. ; Andrulis, Irene L. ; Daly, Mary B. ; John, Esther M. ; Keegan, Theresa H ; Knight, Julia ; Bradbury, Angela R. ; Kappil, Maya A. ; Gurvich, Irina ; Santella, Regina M. ; Terry, Mary Beth. / Correlation of DNA methylation levels in blood and saliva DNA in young girls of the LEGACY girls study. In: Epigenetics. 2014 ; Vol. 9, No. 7. pp. 929-933.
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abstract = "Many epidemiologic studies of environmental exposures and disease susceptibility measure DNA methylation in white blood cells (WBC). Some studies are also starting to use saliva DNA as it is usually more readily available in large epidemiologic studies. However, little is known about the correlation of methylation between WBC and saliva DNA. We examined DNA methylation in three repetitive elements, Sat2, Alu, and LINE-1, and in four CpG sites, including AHRR (cg23576855, cg05575921), cg05951221 at 2q37.1, and cg11924019 at CYP1A1, in 57 girls aged 6-15 years with blood and saliva collected on the same day. We measured all DNA methylation markers by bisulfite-pyrosequencing, except for Sat2 and Alu, which were measured by the MethyLight assay. Methylation levels measured in saliva DNA were lower than those in WBC DNA, with differences ranging from 2.8{\%} for Alu to 14.1{\%} for cg05575921. Methylation levels for the three repetitive elements measured in saliva DNA were all positively correlated with those in WBC DNA. However, there was a wide range in the Spearman correlations, with the smallest correlation found for Alu (0.24) and the strongest correlation found for LINE-1 (0.73). Spearman correlations for cg05575921, cg05951221, and cg11924019 were 0.33, 0.42, and 0.79, respectively. If these findings are replicated in larger studies, they suggest that, for selected methylation markers (e.g., LINE-1), methylation levels may be highly correlated between blood and saliva, while for others methylation markers, the levels may be more tissue specific. Thus, in studies that differ by DNA source, each interrogated site should be separately examined in order to evaluate the correlation in DNA methylation levels across DNA sources.",
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