Correlation between α-amylase gene expression and seedling vigor in rice

Erik E. Karrer, John M. Chandler, Majid R. Foolad, Raymond L. Rodriguez

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

This report examines the relationship between seedling vigor, α-amylase enzyme activity and α-amylase mRNA accumulation in ten varieties of rice (Oryza sativa L.) grown at two temperatures (15°C and 30°C). A significant, positive correlation was observed between seedling vigor, α-amylase enzyme activity, and the accumulation of mRNA from one rice α-amylase gene (RAmy1A) at both temperature regimens. The results of this study support previous experiments which have correlated α-amylase enzyme activity to seedling vigor. We have extended this correlation to the expression of one of ten genes that comprise the rice α-amylase multigene family. These results suggest that the expression of α-amylase gene RAmy1A is an important, and possibly rate-limiting factor in determining seedling vigor in rice.

Original languageEnglish (US)
Pages (from-to)163-169
Number of pages7
JournalEuphytica
Volume66
Issue number3
DOIs
StatePublished - Jan 1992

Fingerprint

Amylases
Seedlings
amylases
vigor
Gene Expression
rice
gene expression
seedlings
enzyme activity
Enzymes
Messenger RNA
Temperature
genes
Multigene Family
Oryza
multigene family
Genes
Oryza sativa
temperature

Keywords

  • α-amylase enzyme activity
  • α-amylase mRNA levels
  • cold temperature
  • differential gene expression
  • Oryza sativa
  • rice

ASJC Scopus subject areas

  • Plant Science
  • Horticulture
  • Agronomy and Crop Science

Cite this

Karrer, E. E., Chandler, J. M., Foolad, M. R., & Rodriguez, R. L. (1992). Correlation between α-amylase gene expression and seedling vigor in rice. Euphytica, 66(3), 163-169. https://doi.org/10.1007/BF00025299

Correlation between α-amylase gene expression and seedling vigor in rice. / Karrer, Erik E.; Chandler, John M.; Foolad, Majid R.; Rodriguez, Raymond L.

In: Euphytica, Vol. 66, No. 3, 01.1992, p. 163-169.

Research output: Contribution to journalArticle

Karrer, EE, Chandler, JM, Foolad, MR & Rodriguez, RL 1992, 'Correlation between α-amylase gene expression and seedling vigor in rice', Euphytica, vol. 66, no. 3, pp. 163-169. https://doi.org/10.1007/BF00025299
Karrer, Erik E. ; Chandler, John M. ; Foolad, Majid R. ; Rodriguez, Raymond L. / Correlation between α-amylase gene expression and seedling vigor in rice. In: Euphytica. 1992 ; Vol. 66, No. 3. pp. 163-169.
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