TY - JOUR
T1 - Corneal storage medium preservation with defensins
AU - Schwab, I. R.
AU - Dries, D.
AU - Cullor, James S
AU - Smith, W.
AU - Mannis, Mark J
AU - Reid, T.
AU - Murphy, Christopher J
PY - 1992
Y1 - 1992
N2 - We used a synthetic defensin (rabbit neutrophil peptide-1; NP-1)as a microbicide in a corneal storage medium. Optisol without antimicrobial compounds. We established growth curves in Optisol for Staphylococcus aureus. Streptococcus pneumoniae, and Pseudomonas aeruginosa. Each organism was evaluated separately at 4°C, 23°C, and 37°C in Optisol with NP-1 at each of four different concentrations including 1, 10, 100, and 200 μg/ml. When the three organisms were evaluated in Optisol containing NP-1, we found that a concentration of 200 μg/ml of NP-1 successfully killed 99.9% (the limits of our assay) of all three organisms at all temperatures tested. We conclude that NP-1 exhibits promise as a nonantibiotic preservative agent in corneal storage media, since it was effective in killing organisms at all temperatures, including 4°C.
AB - We used a synthetic defensin (rabbit neutrophil peptide-1; NP-1)as a microbicide in a corneal storage medium. Optisol without antimicrobial compounds. We established growth curves in Optisol for Staphylococcus aureus. Streptococcus pneumoniae, and Pseudomonas aeruginosa. Each organism was evaluated separately at 4°C, 23°C, and 37°C in Optisol with NP-1 at each of four different concentrations including 1, 10, 100, and 200 μg/ml. When the three organisms were evaluated in Optisol containing NP-1, we found that a concentration of 200 μg/ml of NP-1 successfully killed 99.9% (the limits of our assay) of all three organisms at all temperatures tested. We conclude that NP-1 exhibits promise as a nonantibiotic preservative agent in corneal storage media, since it was effective in killing organisms at all temperatures, including 4°C.
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M3 - Article
C2 - 1424662
AN - SCOPUS:0026665288
VL - 11
SP - 370
EP - 375
JO - Cornea
JF - Cornea
SN - 0277-3740
IS - 5
ER -