Copper deficiency and arachidonic acid enhance insulin secretion in isolated pancreatic islets from lean (FaFa) Zucker rats

Gary D. Miller, Carl L Keen, Judith S. Stern, Janet Y. Uriu-Hare

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6 Scopus citations

Abstract

Based on reports that copper deficiency can result in altered arachidonic acid (AA) metabolism and the knowledge that AA can modulate insulin secretion, we investigated the interaction of copper deficiency and AA on glucose-stimulated insulin secretion by using isolated islets. Lean male (Fa/Fa) Zucker rats were fed either a copper-adequate (Cont) or low-Cu (Cu Defic) diet (8.7 vs. 0.5 μg copper/g diet) for 5 weeks. Pancreatic islets were isolated and incubated in medium containing 3.3, 8.3, or 16.7 mM glucose with or without 100 μM AA. Insulin secretion was measured after either 5 (phase I) or 45 (phase II) min of incubation. In the absence of AA, islets from Cu Defic rats secreted more insulin during phase I than those from Cont rats; phase II insulin secretion was similar between the groups. Exogenous AA treatment increased insulin secretion from islets during both phases of insulin secretion, regardless of dietary copper treatment. In the presence of AA, islets from Cu Defic rats secreted more insulin in phase I than did islets from Cont rats at the 3.3 and 8.3 mM glucose levels. Similar findings were made for phase II insulin secretion. Consistent with this, Cu Defic rats tended to have higher-plasma insulin levels than Cont rats (472.6 vs. 199.6 pM). In summary, Cu deficiency increases insulin release in isolated islets; this response is particularly pronounced when AA is present in the incubation medium.

Original languageEnglish (US)
Pages (from-to)390-396
Number of pages7
JournalPancreas
Volume17
Issue number4
StatePublished - 1998

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Keywords

  • Arachidonic acid
  • Copper deficiency
  • Insulin secretion
  • Isolated islets
  • Zucker rats

ASJC Scopus subject areas

  • Gastroenterology
  • Endocrinology

Cite this

Miller, G. D., Keen, C. L., Stern, J. S., & Uriu-Hare, J. Y. (1998). Copper deficiency and arachidonic acid enhance insulin secretion in isolated pancreatic islets from lean (FaFa) Zucker rats. Pancreas, 17(4), 390-396.