Copper chelates as probes of biological systems: Stable copper complexes with a macrocyclic bifunctional chelating agent

Min K. Moi, Claude F. Meares, Michael J. McCall, William C. Cole, Sally J. DeNardo

Research output: Contribution to journalArticle

184 Scopus citations

Abstract

Monoclonal antibodies tagged with chelated metal ions have numerous potential applications. Here we report the synthesis of a new "bifunctional" metal chelator, 6(p-bromoacetamidobenzyl)-1,4,8,11-tetraazacyclotetradecane-N,N′,N″,N‴-tetraacetic acid, which can be covalently attached to proteins and which binds copper stably in human serum under physiological conditions. In contrast, other reagents based on ethylenediaminetetraacetic acid or diethylenetriaminepentaacetic acid rapidly lose copper to serum albumin under the same conditions.

Original languageEnglish (US)
Pages (from-to)249-253
Number of pages5
JournalAnalytical Biochemistry
Volume148
Issue number1
DOIs
StatePublished - 1985

Keywords

  • copper
  • HPLC, proteins
  • immunoglobulins
  • organic synthesis
  • radioactive
  • radioactivity measurement
  • serum

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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