Coordinate alteration of pp60(c-src) abundance and c-src RNA expression in human neuroblastoma variants

A. Veillette, J. O'Shaughnessy, I. D. Horak, M. A. Israel, D. Yee, N. Rosen, D. J. Fujita, Hsing-Jien Kung, J. L. Biedler, J. B. Bolen

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Abstract

We examined the expression, abundance, and protein kinase activity of pp60(c-src) in two different pairs of genetically indistinguishable cloned human neuroblastoma cell variants which display altered phenotypes as the result of conversion from a neuronal to a non-neuronal phenotype. The results demonstrate that cells which exhibit the neuroblastic (N-type) phenotype possess high levels of pp60(c-src) protein and that one of the two N-type cell lines is capable of expressing the neuronal-specific isoenzyme of pp60(c-src). In contrast, cells which display the substrate-adherent (S-type) phenotype have low levels of pp60(c-src) protein and express exclusively the non-neuronal isoenzyme of pp60(c-src). In all cells examined the abundance of pp60(c-src) was found to be proportional to the steady-state level of c-src RNA. In each case the protein kinase activity of pp60(c-src) was found to be proportional to the abundance of the protein and independent of the ratio of neuronal to non-neuronal species of pp60(c-src).

Original languageEnglish (US)
Pages (from-to)421-427
Number of pages7
JournalOncogene
Volume4
Issue number4
StatePublished - 1989
Externally publishedYes

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ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Veillette, A., O'Shaughnessy, J., Horak, I. D., Israel, M. A., Yee, D., Rosen, N., Fujita, D. J., Kung, H-J., Biedler, J. L., & Bolen, J. B. (1989). Coordinate alteration of pp60(c-src) abundance and c-src RNA expression in human neuroblastoma variants. Oncogene, 4(4), 421-427.