Cooperative Accumulation of Dynein-Dynactin at Microtubule Minus-Ends Drives Microtubule Network Reorganization

Ruensern Tan, Peter J. Foster, Daniel J. Needleman, Richard Mckenney

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cytoplasmic dynein-1 is a minus-end-directed motor protein that transports cargo over long distances and organizes the intracellular microtubule (MT) network. How dynein motor activity is harnessed for these diverse functions remains unknown. Here, we have uncovered a mechanism for how processive dynein-dynactin complexes drive MT-MT sliding, reorganization, and focusing, activities required for mitotic spindle assembly. We find that motors cooperatively accumulate, in limited numbers, at MT minus-ends. Minus-end accumulations drive MT-MT sliding, independent of MT orientation, resulting in the clustering of MT minus-ends. At a mesoscale level, activated dynein-dynactin drives the formation and coalescence of MT asters. Macroscopically, dynein-dynactin activity leads to bulk contraction of millimeter-scale MT networks, suggesting that minus-end accumulations of motors produce network-scale contractile stresses. Our data provide a model for how localized dynein activity is harnessed by cells to produce contractile stresses within the cytoskeleton, for example, during mitotic spindle assembly. Tan et al. demonstrate how individual cytoplasmic dynein motors organize incoherent collections of microtubules into polarity-sorted structures at varying length scales. Dynein cooperatively accumulates into clusters at microtubule minus-ends, reorganizing microtubules exclusively via clusters, thus providing a molecular explanation for dynein's structural role in mitotic spindle assembly.

Original languageEnglish (US)
Pages (from-to)233-247.e4
JournalDevelopmental Cell
Volume44
Issue number2
DOIs
StatePublished - Jan 22 2018

Fingerprint

Dyneins
Microtubules
Cytoplasmic Dyneins
Spindle Apparatus
Dynactin Complex
Drive
Coalescence
Protein Transport
Cytoskeleton
Cluster Analysis

Keywords

  • active matter
  • aster
  • dynactin
  • dynein
  • microtubule
  • minus-end
  • molecular motor
  • spindle

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Cooperative Accumulation of Dynein-Dynactin at Microtubule Minus-Ends Drives Microtubule Network Reorganization. / Tan, Ruensern; Foster, Peter J.; Needleman, Daniel J.; Mckenney, Richard.

In: Developmental Cell, Vol. 44, No. 2, 22.01.2018, p. 233-247.e4.

Research output: Contribution to journalArticle

Tan, Ruensern ; Foster, Peter J. ; Needleman, Daniel J. ; Mckenney, Richard. / Cooperative Accumulation of Dynein-Dynactin at Microtubule Minus-Ends Drives Microtubule Network Reorganization. In: Developmental Cell. 2018 ; Vol. 44, No. 2. pp. 233-247.e4.
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