Conversion potential of marrow cells into lung cells fluctuates with cytokine-induced cell cycle

Mark S. Dooner, Jason M. Aliotta, Jeffrey Pimentel, Gerri J. Dooner, Mehrdad Abedi, Gerald Colvin, Qin Liu, Heinz Ulli Weier, Kevin W. Johnson, Peter J. Quesenberry

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Green fluorescent protein (GFP)-labeled marrow cells transplanted into lethally irradiated mice can be detected in the lungs of transplanted mice and have been shown to express lung-specific proteins while lacking the expression of hematopoietic markers. We have studied marrow cells induced to transit the cell cycle by exposure to interleukin-3 (IL-3), IL-6, IL-11, and Steel factor at different times of culture corresponding to different phases of cell cycle. We have found that marrow cells at the G1 /S interface of the cell cycle have a three-fold increase in cells that assume a nonhematopoietic or pulmonary epithelial cell phenotype and that this increase is no longer seen in late S/G 2. These cells have been characterized as GFP+ CD45 - and GFP+ cytokeratin+. Thus, marrow cells with the capacity to convert into cells with a lung phenotype after transplantation show a reversible increase with cytokine-induced cell cycle transit. Previous studies have shown that the phenotype of bone marrow stem cells fluctuates reversibly as these cells traverse the cell cycle, leading to a continuum model of stem cell regulation. The present study indicates that marrow stem cell production of nonhematopoietic cells also fluctuates on a continuum.

Original languageEnglish (US)
Pages (from-to)207-219
Number of pages13
JournalStem Cells and Development
Volume17
Issue number2
DOIs
StatePublished - Apr 1 2008
Externally publishedYes

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Cell Cycle
Bone Marrow
Cytokines
Lung
Green Fluorescent Proteins
Stem Cells
Phenotype
Interleukin-11
Stem Cell Factor
Interleukin-3
Keratins
Bone Marrow Cells
Interleukin-6
Transplantation
Epithelial Cells
Proteins

ASJC Scopus subject areas

  • Hematology

Cite this

Dooner, M. S., Aliotta, J. M., Pimentel, J., Dooner, G. J., Abedi, M., Colvin, G., ... Quesenberry, P. J. (2008). Conversion potential of marrow cells into lung cells fluctuates with cytokine-induced cell cycle. Stem Cells and Development, 17(2), 207-219. https://doi.org/10.1089/scd.2007.0195

Conversion potential of marrow cells into lung cells fluctuates with cytokine-induced cell cycle. / Dooner, Mark S.; Aliotta, Jason M.; Pimentel, Jeffrey; Dooner, Gerri J.; Abedi, Mehrdad; Colvin, Gerald; Liu, Qin; Weier, Heinz Ulli; Johnson, Kevin W.; Quesenberry, Peter J.

In: Stem Cells and Development, Vol. 17, No. 2, 01.04.2008, p. 207-219.

Research output: Contribution to journalArticle

Dooner, MS, Aliotta, JM, Pimentel, J, Dooner, GJ, Abedi, M, Colvin, G, Liu, Q, Weier, HU, Johnson, KW & Quesenberry, PJ 2008, 'Conversion potential of marrow cells into lung cells fluctuates with cytokine-induced cell cycle', Stem Cells and Development, vol. 17, no. 2, pp. 207-219. https://doi.org/10.1089/scd.2007.0195
Dooner, Mark S. ; Aliotta, Jason M. ; Pimentel, Jeffrey ; Dooner, Gerri J. ; Abedi, Mehrdad ; Colvin, Gerald ; Liu, Qin ; Weier, Heinz Ulli ; Johnson, Kevin W. ; Quesenberry, Peter J. / Conversion potential of marrow cells into lung cells fluctuates with cytokine-induced cell cycle. In: Stem Cells and Development. 2008 ; Vol. 17, No. 2. pp. 207-219.
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