Conventional transmission electron microscopy

Mark Winey, Janet B. Meehl, Eileen T. O'Toole, Thomas H. Giddings

Research output: Contribution to journalReview article

43 Scopus citations

Abstract

Researchers have used transmission electron microscopy (TEM) to make contributions to cell biology for well over 50 years, and TEM continues to be an important technology in our field. We briefly present for the neophyte the components of a TEM-based study, beginning with sample preparation through imaging of the samples. We point out the limitations of TEM and issues to be considered during experimental design. Advanced electron microscopy techniques are listed as well. Finally, we point potential new users of TEM to resources to help launch their project.

Original languageEnglish (US)
Pages (from-to)319-323
Number of pages5
JournalMolecular Biology of the Cell
Volume25
Issue number3
DOIs
StatePublished - Feb 1 2014
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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