Controlling protein activity with ligand-regulated RNA aptamers

Momchilo Vuyisich, Peter A. Beal

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Controlling the activity of a protein is necessary for defining its function in vivo. RNA aptamers are capable of inhibiting proteins with high affinity and specificity, but this effect is not readily reversible. We describe a general method for discovering aptamers that bind and inhibit their target protein, but addition of a specific small molecule disrupts the protein-RNA complex. A SELEX protocol was used to raise RNA aptamers to the DNA repair enzyme, formamidopyrimidine glycosylase (Fpg), and neomycin was employed in each round to dissociate Fpg-bound RNAs. We identified an RNA molecule able to completely inhibit Fpg at 100 nM concentration. Importantly, Fpg activity is recovered by the addition of neomycin. We envision these ligand-regulated aptamers (LIRAs) as valuable tools in the study of biological phenomena in which the timing of molecular events is critical.

Original languageEnglish (US)
Pages (from-to)907-913
Number of pages7
JournalChemistry and Biology
Volume9
Issue number8
DOIs
StatePublished - Aug 2002
Externally publishedYes

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ASJC Scopus subject areas

  • Organic Chemistry

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