Controlled-release components of PZP contraceptive vaccine extend duration of infertility

John W. Turner, Allen T. Rutberg, Ricky E. Naugle, Manpreet A. Kaur, Douglas R. Flanagan, Henk J. Bertschinger, Irwin Liu

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Successful immunocontraception of wildlife relying on repeated access to individuals for boosters has highlighted the need to incorporate primer and booster immunisations into one injection. We have investigated use of controlled-release polymers (lactide-glycolide) in small pellets to provide delayed in vivo delivery of booster porcine zona pellucida (PZP) antigen and adjuvant. This report reviews pellet-making methodology, in vitro testing of controlled-release pellets and in vivo effects of controlled-release PZP vaccine. We assessed 3 different manufacturing approaches for producing reliable, cost-effective pellets: (1) polymer melting and extrusion; (2) solvent evaporation from polymer solution; and (3) punch and die polymer moulding. In vitro testing of release patterns of controlled-release formulations, towards development of a 3-year duration vaccine, provided estimates for in vivo use of pellet preparations. These in vitro studies demonstrated protein release delay up to 22 months using 100% l-lactide or polycaprolactone polymers. For in vivo tests, pellets (1-, 3-, and 12-month release delay) serving as boosters were administered intramuscularly with PZP/adjuvant liquid primer to wild horses (Equus caballus), white-tailed deer (Odocoileus virginanus) and African elephants (Loxodonta africana). Horse field studies assessed fertility via offspring counts and/or faecal-hormone pregnancy testing. Treatment decreased fertility 5.3-9.3-fold in Year 1 and 3.6-fold in Year 2. In preliminary testing in deer, offspring counts revealed treatment-associated fertility reduction of 7.1-fold Year 1 and 3.3-fold Year 2. In elephants, treatment elevated anti-PZP titres 4.5-6.9-fold from pretreatment (no fertility data).

Original languageEnglish (US)
Pages (from-to)555-562
Number of pages8
JournalWildlife Research
Volume35
Issue number6
DOIs
StatePublished - Oct 28 2008

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contraceptive vaccines
infertility
zona pellucida
vaccine
pellets
polymer
fertility
polymers
fold
swine
duration
elephant
horse
Loxodonta africana
deer
horses
immunocontraception
testing
adjuvants
Odocoileus

ASJC Scopus subject areas

  • Management, Monitoring, Policy and Law
  • Ecology, Evolution, Behavior and Systematics

Cite this

Turner, J. W., Rutberg, A. T., Naugle, R. E., Kaur, M. A., Flanagan, D. R., Bertschinger, H. J., & Liu, I. (2008). Controlled-release components of PZP contraceptive vaccine extend duration of infertility. Wildlife Research, 35(6), 555-562. https://doi.org/10.1071/WR07159

Controlled-release components of PZP contraceptive vaccine extend duration of infertility. / Turner, John W.; Rutberg, Allen T.; Naugle, Ricky E.; Kaur, Manpreet A.; Flanagan, Douglas R.; Bertschinger, Henk J.; Liu, Irwin.

In: Wildlife Research, Vol. 35, No. 6, 28.10.2008, p. 555-562.

Research output: Contribution to journalArticle

Turner, JW, Rutberg, AT, Naugle, RE, Kaur, MA, Flanagan, DR, Bertschinger, HJ & Liu, I 2008, 'Controlled-release components of PZP contraceptive vaccine extend duration of infertility', Wildlife Research, vol. 35, no. 6, pp. 555-562. https://doi.org/10.1071/WR07159
Turner JW, Rutberg AT, Naugle RE, Kaur MA, Flanagan DR, Bertschinger HJ et al. Controlled-release components of PZP contraceptive vaccine extend duration of infertility. Wildlife Research. 2008 Oct 28;35(6):555-562. https://doi.org/10.1071/WR07159
Turner, John W. ; Rutberg, Allen T. ; Naugle, Ricky E. ; Kaur, Manpreet A. ; Flanagan, Douglas R. ; Bertschinger, Henk J. ; Liu, Irwin. / Controlled-release components of PZP contraceptive vaccine extend duration of infertility. In: Wildlife Research. 2008 ; Vol. 35, No. 6. pp. 555-562.
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