Control of respiration and bioenergetics during muscle contraction

Youngran Chung, Paul A. Molé, Napapon Sailasuta, Khanh Tran Tuan, Ralph Hurd, Thomas Jue

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

H-NMR experiments have determined intracellular O 2 consumption (V̇ O2) with oxymyoglobin (MbO 2) desaturation kinetics in human calf muscle during plantar flexion exercise at 0.75, 0.92, and 1.17 Hz with a constant load. At the onset of muscle contraction, myoglobin (Mb) desaturates rapidly. The desaturation rate constant of ∼30 s reflects the intracellular V̇ O2. Although Mb desaturates quickly with a similar time constant at all workload levels, its final steady-state level differs. As work increases, the final steady-state cellular P O2 decreases progressively. After Mb desaturation has reached a steady state, however, V̇ O2 continues to rise. On the basis of current respiratory control models, the analysis in the present report reveals two distinct V̇ O2 phases: an ADP-independent phase at the onset of contraction and an ADP-dependent phase after Mb has reached a steady state. In contrast to the accepted view, the initial intracellular V̇ O2 shows that oxidative phosphorylation can support up to 36% of the energy cost, a significantly higher fraction than expected. Partitioning of the energy flux shows that a 31% nonoxidative component exists and responds to the dynamic energy utilization-restoration cycle (which lasts for only milliseconds) as postulated in the glycogen shunt theory. The present study offers perspectives on the regulation of respiration, bioenergetics, and Mb function during muscle contraction.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume288
Issue number3 57-3
DOIs
StatePublished - Mar 2005

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Myoglobin
Muscle Contraction
Energy Metabolism
Muscle
Respiration
Adenosine Diphosphate
Oxidative Phosphorylation
Workload
Glycogen
Restoration
Rate constants
Energy utilization
Nuclear magnetic resonance
Fluxes
Costs and Cost Analysis
Muscles
Kinetics
Costs
Experiments

Keywords

  • Exercise
  • Glycogen
  • Myoglobin
  • Nuclear magnetic resonance
  • Oxygen

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Control of respiration and bioenergetics during muscle contraction. / Chung, Youngran; Molé, Paul A.; Sailasuta, Napapon; Tuan, Khanh Tran; Hurd, Ralph; Jue, Thomas.

In: American Journal of Physiology - Cell Physiology, Vol. 288, No. 3 57-3, 03.2005.

Research output: Contribution to journalArticle

Chung, Youngran ; Molé, Paul A. ; Sailasuta, Napapon ; Tuan, Khanh Tran ; Hurd, Ralph ; Jue, Thomas. / Control of respiration and bioenergetics during muscle contraction. In: American Journal of Physiology - Cell Physiology. 2005 ; Vol. 288, No. 3 57-3.
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