Control of Protein Structure and Function through Surface Recognition by Tailored Nanoparticle Scaffolds

Rui Hong, Nicholas O Fischer, Ayush Verma, Catherine M. Goodman, Todd Emrick, Vincent M. Rotello

Research output: Contribution to journalArticle

244 Scopus citations

Abstract

Thioalkyl and thioalkylated oligo(ethylene glycol) (OEG) ligands with chain-end functionality were used to fabricate water-soluble CdSe nanoparticle scaffolds. Surface recognition of chymotrypsin (ChT) was achieved using these functionalized nanoparticle scaffolds, with three levels of interaction demonstrated: no interaction (OEG terminated with hydroxyl group), inhibition with denaturation (carboxylate-terminated thioalkyl ligands), and inhibition with retention of structure (carboxylate-terminated OEG). The latter process was reversible upon an increase in ionic strength, with essentially complete restoration of enzymatic activity.

Original languageEnglish (US)
Pages (from-to)739-743
Number of pages5
JournalJournal of the American Chemical Society
Volume126
Issue number3
DOIs
StatePublished - Jan 28 2004
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)

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