Control of Protein Structure and Function through Surface Recognition by Tailored Nanoparticle Scaffolds

Rui Hong; Nicholas O Fischer; Ayush Verma; Catherine M. Goodman; Todd Emrick; Vincent M. Rotello

doi:10.1021/ja037470o

Control of Protein Structure and Function through Surface Recognition by Tailored Nanoparticle Scaffolds

Rui Hong, Nicholas O Fischer, Ayush Verma, Catherine M. Goodman, Todd Emrick, Vincent M. Rotello

Research output: Contribution to journal › Article › peer-review

250 Scopus citations

Abstract

Thioalkyl and thioalkylated oligo(ethylene glycol) (OEG) ligands with chain-end functionality were used to fabricate water-soluble CdSe nanoparticle scaffolds. Surface recognition of chymotrypsin (ChT) was achieved using these functionalized nanoparticle scaffolds, with three levels of interaction demonstrated: no interaction (OEG terminated with hydroxyl group), inhibition with denaturation (carboxylate-terminated thioalkyl ligands), and inhibition with retention of structure (carboxylate-terminated OEG). The latter process was reversible upon an increase in ionic strength, with essentially complete restoration of enzymatic activity.

Original language	English (US)
Pages (from-to)	739-743
Number of pages	5
Journal	Journal of the American Chemical Society
Volume	126
Issue number	3
DOIs	https://doi.org/10.1021/ja037470o
State	Published - Jan 28 2004
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)

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abstract = "Thioalkyl and thioalkylated oligo(ethylene glycol) (OEG) ligands with chain-end functionality were used to fabricate water-soluble CdSe nanoparticle scaffolds. Surface recognition of chymotrypsin (ChT) was achieved using these functionalized nanoparticle scaffolds, with three levels of interaction demonstrated: no interaction (OEG terminated with hydroxyl group), inhibition with denaturation (carboxylate-terminated thioalkyl ligands), and inhibition with retention of structure (carboxylate-terminated OEG). The latter process was reversible upon an increase in ionic strength, with essentially complete restoration of enzymatic activity.",

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AU - Hong, Rui

AU - Fischer, Nicholas O

AU - Verma, Ayush

AU - Goodman, Catherine M.

AU - Emrick, Todd

AU - Rotello, Vincent M.

PY - 2004/1/28

Y1 - 2004/1/28

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AB - Thioalkyl and thioalkylated oligo(ethylene glycol) (OEG) ligands with chain-end functionality were used to fabricate water-soluble CdSe nanoparticle scaffolds. Surface recognition of chymotrypsin (ChT) was achieved using these functionalized nanoparticle scaffolds, with three levels of interaction demonstrated: no interaction (OEG terminated with hydroxyl group), inhibition with denaturation (carboxylate-terminated thioalkyl ligands), and inhibition with retention of structure (carboxylate-terminated OEG). The latter process was reversible upon an increase in ionic strength, with essentially complete restoration of enzymatic activity.

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