Contribution of the cytoskeleton to the compressive properties and recovery behavior of single cells

Gidon Ofek, Dena C. Wiltz, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The cytoskeleton is known to play an important role in the biomechanical nature and structure of cells, but its particular function in compressive characteristics has not yet been fully examined. This study focused on the contribution of the main three cytoskeletal elements to the bulk compressive stiffness (as measured by the compressive modulus), volumetric or apparent compressibility changes (as further indicated by apparent Poisson's ratio), and recovery behavior of individual chondrocytes. Before mechanical testing, cytochalasin D, acrylamide, or colchicine was used to disrupt actin microfilaments, intermediate filaments, or microtubules, respectively. Cells were subjected to a range of compressive strains and allowed to recover to equilibrium. Analysis of the video recording for each mechanical event yielded relevant compressive properties and recovery characteristics related to the specific cytoskeletal disrupting agent and as a function of applied axial strain. Inhibition of actin microfilaments had the greatest effect on bulk compressive stiffness (∼50% decrease compared to control). Meanwhile, intermediate filaments and microtubules were each found to play an integral role in either the diminution (compressibility) or retention (incompressibility) of original cell volume during compression. In addition, microtubule disruption had the largest effect on the "critical strain threshold" in cellular mechanical behavior (33% decrease compared to control), as well as the characteristic time for recovery (∼100% increase compared to control). Elucidating the role of the cytoskeleton in the compressive biomechanical behavior of single cells is an important step toward understanding the basis of mechanotransduction and the etiology of cellular disease processes.

Original languageEnglish (US)
Pages (from-to)1873-1882
Number of pages10
JournalBiophysical Journal
Volume97
Issue number7
DOIs
StatePublished - Oct 7 2009

Fingerprint

Cytoskeleton
Microtubules
Intermediate Filaments
Actin Cytoskeleton
Cellular Mechanotransduction
Cytochalasin D
Video Recording
Acrylamide
Colchicine
Chondrocytes
Cell Size

ASJC Scopus subject areas

  • Biophysics

Cite this

Contribution of the cytoskeleton to the compressive properties and recovery behavior of single cells. / Ofek, Gidon; Wiltz, Dena C.; Athanasiou, Kyriacos A.

In: Biophysical Journal, Vol. 97, No. 7, 07.10.2009, p. 1873-1882.

Research output: Contribution to journalArticle

Ofek, Gidon ; Wiltz, Dena C. ; Athanasiou, Kyriacos A. / Contribution of the cytoskeleton to the compressive properties and recovery behavior of single cells. In: Biophysical Journal. 2009 ; Vol. 97, No. 7. pp. 1873-1882.
@article{d9e24421530c438695447fe5927a00ed,
title = "Contribution of the cytoskeleton to the compressive properties and recovery behavior of single cells",
abstract = "The cytoskeleton is known to play an important role in the biomechanical nature and structure of cells, but its particular function in compressive characteristics has not yet been fully examined. This study focused on the contribution of the main three cytoskeletal elements to the bulk compressive stiffness (as measured by the compressive modulus), volumetric or apparent compressibility changes (as further indicated by apparent Poisson's ratio), and recovery behavior of individual chondrocytes. Before mechanical testing, cytochalasin D, acrylamide, or colchicine was used to disrupt actin microfilaments, intermediate filaments, or microtubules, respectively. Cells were subjected to a range of compressive strains and allowed to recover to equilibrium. Analysis of the video recording for each mechanical event yielded relevant compressive properties and recovery characteristics related to the specific cytoskeletal disrupting agent and as a function of applied axial strain. Inhibition of actin microfilaments had the greatest effect on bulk compressive stiffness (∼50{\%} decrease compared to control). Meanwhile, intermediate filaments and microtubules were each found to play an integral role in either the diminution (compressibility) or retention (incompressibility) of original cell volume during compression. In addition, microtubule disruption had the largest effect on the {"}critical strain threshold{"} in cellular mechanical behavior (33{\%} decrease compared to control), as well as the characteristic time for recovery (∼100{\%} increase compared to control). Elucidating the role of the cytoskeleton in the compressive biomechanical behavior of single cells is an important step toward understanding the basis of mechanotransduction and the etiology of cellular disease processes.",
author = "Gidon Ofek and Wiltz, {Dena C.} and Athanasiou, {Kyriacos A.}",
year = "2009",
month = "10",
day = "7",
doi = "10.1016/j.bpj.2009.07.050",
language = "English (US)",
volume = "97",
pages = "1873--1882",
journal = "Biophysical Journal",
issn = "0006-3495",
publisher = "Biophysical Society",
number = "7",

}

TY - JOUR

T1 - Contribution of the cytoskeleton to the compressive properties and recovery behavior of single cells

AU - Ofek, Gidon

AU - Wiltz, Dena C.

AU - Athanasiou, Kyriacos A.

PY - 2009/10/7

Y1 - 2009/10/7

N2 - The cytoskeleton is known to play an important role in the biomechanical nature and structure of cells, but its particular function in compressive characteristics has not yet been fully examined. This study focused on the contribution of the main three cytoskeletal elements to the bulk compressive stiffness (as measured by the compressive modulus), volumetric or apparent compressibility changes (as further indicated by apparent Poisson's ratio), and recovery behavior of individual chondrocytes. Before mechanical testing, cytochalasin D, acrylamide, or colchicine was used to disrupt actin microfilaments, intermediate filaments, or microtubules, respectively. Cells were subjected to a range of compressive strains and allowed to recover to equilibrium. Analysis of the video recording for each mechanical event yielded relevant compressive properties and recovery characteristics related to the specific cytoskeletal disrupting agent and as a function of applied axial strain. Inhibition of actin microfilaments had the greatest effect on bulk compressive stiffness (∼50% decrease compared to control). Meanwhile, intermediate filaments and microtubules were each found to play an integral role in either the diminution (compressibility) or retention (incompressibility) of original cell volume during compression. In addition, microtubule disruption had the largest effect on the "critical strain threshold" in cellular mechanical behavior (33% decrease compared to control), as well as the characteristic time for recovery (∼100% increase compared to control). Elucidating the role of the cytoskeleton in the compressive biomechanical behavior of single cells is an important step toward understanding the basis of mechanotransduction and the etiology of cellular disease processes.

AB - The cytoskeleton is known to play an important role in the biomechanical nature and structure of cells, but its particular function in compressive characteristics has not yet been fully examined. This study focused on the contribution of the main three cytoskeletal elements to the bulk compressive stiffness (as measured by the compressive modulus), volumetric or apparent compressibility changes (as further indicated by apparent Poisson's ratio), and recovery behavior of individual chondrocytes. Before mechanical testing, cytochalasin D, acrylamide, or colchicine was used to disrupt actin microfilaments, intermediate filaments, or microtubules, respectively. Cells were subjected to a range of compressive strains and allowed to recover to equilibrium. Analysis of the video recording for each mechanical event yielded relevant compressive properties and recovery characteristics related to the specific cytoskeletal disrupting agent and as a function of applied axial strain. Inhibition of actin microfilaments had the greatest effect on bulk compressive stiffness (∼50% decrease compared to control). Meanwhile, intermediate filaments and microtubules were each found to play an integral role in either the diminution (compressibility) or retention (incompressibility) of original cell volume during compression. In addition, microtubule disruption had the largest effect on the "critical strain threshold" in cellular mechanical behavior (33% decrease compared to control), as well as the characteristic time for recovery (∼100% increase compared to control). Elucidating the role of the cytoskeleton in the compressive biomechanical behavior of single cells is an important step toward understanding the basis of mechanotransduction and the etiology of cellular disease processes.

UR - http://www.scopus.com/inward/record.url?scp=70349665440&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349665440&partnerID=8YFLogxK

U2 - 10.1016/j.bpj.2009.07.050

DO - 10.1016/j.bpj.2009.07.050

M3 - Article

VL - 97

SP - 1873

EP - 1882

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

IS - 7

ER -