Contribution of Salmonella typhimurium virulence factors to diarrheal disease in calves

Renee M Tsolis, L. Garry Adams, Thomas A. Ficht, Andreas J Baumler

Research output: Contribution to journalArticle

229 Citations (Scopus)

Abstract

Limited knowledge is available about the virulence mechanisms responsible for diarrheal disease caused by Salmonella typhimurium. To assess the contribution to diarrheal disease of virulence determinants identified in models of infection, we tested a collection of S. typhimurium mutants for their ability to cause enteritis in calves. S. typhimurium strains carrying mutations in the virulence plasmid (spvR), Salmonella pathogenicity island 2 (SPI-2) (spiB), or SPI-5 (sopB) caused mortality and acute diarrhea in calves. An S. typhimurium rfaJ mutant, which is defective for lipopolysaccharide outer core biosynthesis, was of intermediate virulence. Mutations in SPI-1 (hilA and prgH) or aroA markedly reduced virulence and the severity of diarrhea. Furthermore, histopathological examination of calves infected with SPI-1 or aroA mutants revealed a marked reduction or absence of intestinal lesions. These data suggest that virulence factors, such as SPI-1, which are required during intestinal colonization are more important for pathogenicity in calves than are genes required during the systemic phase of S. typhimurium infection, including SPI-2 or the spv operon. This is in contrast to the degree of attenuation caused by these mutations in the mouse.

Original languageEnglish (US)
Pages (from-to)4879-4885
Number of pages7
JournalInfection and Immunity
Volume67
Issue number9
StatePublished - 1999
Externally publishedYes

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Virulence Factors
Salmonella typhimurium
Virulence
Genomic Islands
Salmonella
Mutation
Diarrhea
Enteritis
Salmonella Infections
Operon
Lipopolysaccharides
Plasmids
Mortality
Infection
Genes

ASJC Scopus subject areas

  • Immunology

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Contribution of Salmonella typhimurium virulence factors to diarrheal disease in calves. / Tsolis, Renee M; Adams, L. Garry; Ficht, Thomas A.; Baumler, Andreas J.

In: Infection and Immunity, Vol. 67, No. 9, 1999, p. 4879-4885.

Research output: Contribution to journalArticle

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