Contrasting proteome biology and functional heterogeneity of the 20 S proteasome complexes in mammalian tissues

Aldrin V Gomes, Glen W. Young, Yueju Wang, Chenggong Zong, Mansoureh Eghbali, Oliver Drewa, Haojie Lu, Enrico Stefani, Pep Ping, Peipei Ping

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The 20 S proteasome complexes are major contributors to the intracellular protein degradation machinery in mammalian cells. Systematic administration of proteasome inhibitors to combat disease (e.g. cancer) has resulted in positive outcomes as well as adversary effects. The latter was attributed to, at least in part, a lack of understanding in the organ-specific responses to inhibitors and the potential diversity of proteomes of these complexes in different tissues. Accordingly, we conducted a proteomic study to characterize the 20 S proteasome complexes and their postulated organ-specific responses in the heart and liver. The cardiac and hepatic 20 S proteasomes were isolated from the same mouse strain with identical genetic background. We examined the molecular composition, complex assembly, post-translational modifications and associating partners of these proteasome complexes. Our results revealed an organ-specific molecular organization of the 20 S proteasomes with distinguished patterns of post-translational modifications as well as unique complex assembly characteristics. Furthermore, the proteome diversities are concomitant with a functional heterogeneity of the proteolytic patterns exhibited by these two organs. In particular, the heart and liver displayed distinct activity profiles to two proteasome inhibitors, epoxomicin and Z-Pro-Nle-Asp-H. Finally, the heart and liver demonstrated contrasting regulatory mechanisms from the associating partners of these proteasomes. The functional heterogeneity of the mammalian 20 S proteasome complexes underscores the concept of divergent proteomes among organs in the context of an identical genome.

Original languageEnglish (US)
Pages (from-to)302-315
Number of pages14
JournalMolecular and Cellular Proteomics
Volume8
Issue number2
DOIs
StatePublished - Feb 2009

Fingerprint

Proteasome Endopeptidase Complex
Proteome
Tissue
Liver
Proteasome Inhibitors
Post Translational Protein Processing
Proteomics
Proteolysis
Machinery
Genes
Cells
Genome
Degradation
Chemical analysis
Neoplasms
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Cite this

Contrasting proteome biology and functional heterogeneity of the 20 S proteasome complexes in mammalian tissues. / Gomes, Aldrin V; Young, Glen W.; Wang, Yueju; Zong, Chenggong; Eghbali, Mansoureh; Drewa, Oliver; Lu, Haojie; Stefani, Enrico; Ping, Pep; Ping, Peipei.

In: Molecular and Cellular Proteomics, Vol. 8, No. 2, 02.2009, p. 302-315.

Research output: Contribution to journalArticle

Gomes, AV, Young, GW, Wang, Y, Zong, C, Eghbali, M, Drewa, O, Lu, H, Stefani, E, Ping, P & Ping, P 2009, 'Contrasting proteome biology and functional heterogeneity of the 20 S proteasome complexes in mammalian tissues', Molecular and Cellular Proteomics, vol. 8, no. 2, pp. 302-315. https://doi.org/10.1074/mcp.M800058-MCP200
Gomes, Aldrin V ; Young, Glen W. ; Wang, Yueju ; Zong, Chenggong ; Eghbali, Mansoureh ; Drewa, Oliver ; Lu, Haojie ; Stefani, Enrico ; Ping, Pep ; Ping, Peipei. / Contrasting proteome biology and functional heterogeneity of the 20 S proteasome complexes in mammalian tissues. In: Molecular and Cellular Proteomics. 2009 ; Vol. 8, No. 2. pp. 302-315.
@article{0e0e8c5d6e284bcfa21b64eb5f8da2c4,
title = "Contrasting proteome biology and functional heterogeneity of the 20 S proteasome complexes in mammalian tissues",
abstract = "The 20 S proteasome complexes are major contributors to the intracellular protein degradation machinery in mammalian cells. Systematic administration of proteasome inhibitors to combat disease (e.g. cancer) has resulted in positive outcomes as well as adversary effects. The latter was attributed to, at least in part, a lack of understanding in the organ-specific responses to inhibitors and the potential diversity of proteomes of these complexes in different tissues. Accordingly, we conducted a proteomic study to characterize the 20 S proteasome complexes and their postulated organ-specific responses in the heart and liver. The cardiac and hepatic 20 S proteasomes were isolated from the same mouse strain with identical genetic background. We examined the molecular composition, complex assembly, post-translational modifications and associating partners of these proteasome complexes. Our results revealed an organ-specific molecular organization of the 20 S proteasomes with distinguished patterns of post-translational modifications as well as unique complex assembly characteristics. Furthermore, the proteome diversities are concomitant with a functional heterogeneity of the proteolytic patterns exhibited by these two organs. In particular, the heart and liver displayed distinct activity profiles to two proteasome inhibitors, epoxomicin and Z-Pro-Nle-Asp-H. Finally, the heart and liver demonstrated contrasting regulatory mechanisms from the associating partners of these proteasomes. The functional heterogeneity of the mammalian 20 S proteasome complexes underscores the concept of divergent proteomes among organs in the context of an identical genome.",
author = "Gomes, {Aldrin V} and Young, {Glen W.} and Yueju Wang and Chenggong Zong and Mansoureh Eghbali and Oliver Drewa and Haojie Lu and Enrico Stefani and Pep Ping and Peipei Ping",
year = "2009",
month = "2",
doi = "10.1074/mcp.M800058-MCP200",
language = "English (US)",
volume = "8",
pages = "302--315",
journal = "Molecular and Cellular Proteomics",
issn = "1535-9476",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "2",

}

TY - JOUR

T1 - Contrasting proteome biology and functional heterogeneity of the 20 S proteasome complexes in mammalian tissues

AU - Gomes, Aldrin V

AU - Young, Glen W.

AU - Wang, Yueju

AU - Zong, Chenggong

AU - Eghbali, Mansoureh

AU - Drewa, Oliver

AU - Lu, Haojie

AU - Stefani, Enrico

AU - Ping, Pep

AU - Ping, Peipei

PY - 2009/2

Y1 - 2009/2

N2 - The 20 S proteasome complexes are major contributors to the intracellular protein degradation machinery in mammalian cells. Systematic administration of proteasome inhibitors to combat disease (e.g. cancer) has resulted in positive outcomes as well as adversary effects. The latter was attributed to, at least in part, a lack of understanding in the organ-specific responses to inhibitors and the potential diversity of proteomes of these complexes in different tissues. Accordingly, we conducted a proteomic study to characterize the 20 S proteasome complexes and their postulated organ-specific responses in the heart and liver. The cardiac and hepatic 20 S proteasomes were isolated from the same mouse strain with identical genetic background. We examined the molecular composition, complex assembly, post-translational modifications and associating partners of these proteasome complexes. Our results revealed an organ-specific molecular organization of the 20 S proteasomes with distinguished patterns of post-translational modifications as well as unique complex assembly characteristics. Furthermore, the proteome diversities are concomitant with a functional heterogeneity of the proteolytic patterns exhibited by these two organs. In particular, the heart and liver displayed distinct activity profiles to two proteasome inhibitors, epoxomicin and Z-Pro-Nle-Asp-H. Finally, the heart and liver demonstrated contrasting regulatory mechanisms from the associating partners of these proteasomes. The functional heterogeneity of the mammalian 20 S proteasome complexes underscores the concept of divergent proteomes among organs in the context of an identical genome.

AB - The 20 S proteasome complexes are major contributors to the intracellular protein degradation machinery in mammalian cells. Systematic administration of proteasome inhibitors to combat disease (e.g. cancer) has resulted in positive outcomes as well as adversary effects. The latter was attributed to, at least in part, a lack of understanding in the organ-specific responses to inhibitors and the potential diversity of proteomes of these complexes in different tissues. Accordingly, we conducted a proteomic study to characterize the 20 S proteasome complexes and their postulated organ-specific responses in the heart and liver. The cardiac and hepatic 20 S proteasomes were isolated from the same mouse strain with identical genetic background. We examined the molecular composition, complex assembly, post-translational modifications and associating partners of these proteasome complexes. Our results revealed an organ-specific molecular organization of the 20 S proteasomes with distinguished patterns of post-translational modifications as well as unique complex assembly characteristics. Furthermore, the proteome diversities are concomitant with a functional heterogeneity of the proteolytic patterns exhibited by these two organs. In particular, the heart and liver displayed distinct activity profiles to two proteasome inhibitors, epoxomicin and Z-Pro-Nle-Asp-H. Finally, the heart and liver demonstrated contrasting regulatory mechanisms from the associating partners of these proteasomes. The functional heterogeneity of the mammalian 20 S proteasome complexes underscores the concept of divergent proteomes among organs in the context of an identical genome.

UR - http://www.scopus.com/inward/record.url?scp=61649083080&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=61649083080&partnerID=8YFLogxK

U2 - 10.1074/mcp.M800058-MCP200

DO - 10.1074/mcp.M800058-MCP200

M3 - Article

C2 - 18931337

AN - SCOPUS:61649083080

VL - 8

SP - 302

EP - 315

JO - Molecular and Cellular Proteomics

JF - Molecular and Cellular Proteomics

SN - 1535-9476

IS - 2

ER -