Rat cardiac muscle cells In culture were subjected to perfusion under constant conditions for periods of 8-16 hours. Capillary tubes were used as perfusion chambers to avoid the disadvantages for kinetic studies inherent in most other perfusion chambers. The contraction rates of these cells displayed a temporal response to perfusion which correlated with the age of the cells in culture. After prolonged perfusion with fresh medium, a peaked response in the contraction rate was observed when the perfusion medium was abruptly changed to medium that had been used to support the growth of a dense culture of cardiac cells for 5 days (conditioned medium).
|Original language||English (US)|
|Number of pages||6|
|State||Published - Aug 1967|
ASJC Scopus subject areas
- Drug Discovery
- Ecology, Evolution, Behavior and Systematics