Contraction rate of rat cardiac cells in culture: Responses to prolonged perfusion

Michael A. Savageau, John P. Steward

Research output: Contribution to journalArticle

Abstract

Rat cardiac muscle cells In culture were subjected to perfusion under constant conditions for periods of 8-16 hours. Capillary tubes were used as perfusion chambers to avoid the disadvantages for kinetic studies inherent in most other perfusion chambers. The contraction rates of these cells displayed a temporal response to perfusion which correlated with the age of the cells in culture. After prolonged perfusion with fresh medium, a peaked response in the contraction rate was observed when the perfusion medium was abruptly changed to medium that had been used to support the growth of a dense culture of cardiac cells for 5 days (conditioned medium).

Original languageEnglish (US)
Pages (from-to)159-164
Number of pages6
JournalBioSystems
Volume1
Issue number3
StatePublished - Aug 1967
Externally publishedYes

Fingerprint

Cell culture
Cardiac
contraction
Rats
Contraction
cell culture
Cell Culture Techniques
Perfusion
Capillary tubes
rats
Cell
Conditioned Culture Medium
Muscle
Cells
cells
Cardiac muscle
kinetics
Kinetics
muscle
Tube

ASJC Scopus subject areas

  • Drug Discovery
  • Biotechnology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Contraction rate of rat cardiac cells in culture : Responses to prolonged perfusion. / Savageau, Michael A.; Steward, John P.

In: BioSystems, Vol. 1, No. 3, 08.1967, p. 159-164.

Research output: Contribution to journalArticle

Savageau, MA & Steward, JP 1967, 'Contraction rate of rat cardiac cells in culture: Responses to prolonged perfusion', BioSystems, vol. 1, no. 3, pp. 159-164.
Savageau, Michael A. ; Steward, John P. / Contraction rate of rat cardiac cells in culture : Responses to prolonged perfusion. In: BioSystems. 1967 ; Vol. 1, No. 3. pp. 159-164.
@article{af89b31c7ce34fa7a07c4dfd5174da23,
title = "Contraction rate of rat cardiac cells in culture: Responses to prolonged perfusion",
abstract = "Rat cardiac muscle cells In culture were subjected to perfusion under constant conditions for periods of 8-16 hours. Capillary tubes were used as perfusion chambers to avoid the disadvantages for kinetic studies inherent in most other perfusion chambers. The contraction rates of these cells displayed a temporal response to perfusion which correlated with the age of the cells in culture. After prolonged perfusion with fresh medium, a peaked response in the contraction rate was observed when the perfusion medium was abruptly changed to medium that had been used to support the growth of a dense culture of cardiac cells for 5 days (conditioned medium).",
author = "Savageau, {Michael A.} and Steward, {John P.}",
year = "1967",
month = "8",
language = "English (US)",
volume = "1",
pages = "159--164",
journal = "BioSystems",
issn = "0303-2647",
publisher = "Elsevier Ireland Ltd",
number = "3",

}

TY - JOUR

T1 - Contraction rate of rat cardiac cells in culture

T2 - Responses to prolonged perfusion

AU - Savageau, Michael A.

AU - Steward, John P.

PY - 1967/8

Y1 - 1967/8

N2 - Rat cardiac muscle cells In culture were subjected to perfusion under constant conditions for periods of 8-16 hours. Capillary tubes were used as perfusion chambers to avoid the disadvantages for kinetic studies inherent in most other perfusion chambers. The contraction rates of these cells displayed a temporal response to perfusion which correlated with the age of the cells in culture. After prolonged perfusion with fresh medium, a peaked response in the contraction rate was observed when the perfusion medium was abruptly changed to medium that had been used to support the growth of a dense culture of cardiac cells for 5 days (conditioned medium).

AB - Rat cardiac muscle cells In culture were subjected to perfusion under constant conditions for periods of 8-16 hours. Capillary tubes were used as perfusion chambers to avoid the disadvantages for kinetic studies inherent in most other perfusion chambers. The contraction rates of these cells displayed a temporal response to perfusion which correlated with the age of the cells in culture. After prolonged perfusion with fresh medium, a peaked response in the contraction rate was observed when the perfusion medium was abruptly changed to medium that had been used to support the growth of a dense culture of cardiac cells for 5 days (conditioned medium).

UR - http://www.scopus.com/inward/record.url?scp=49949133658&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=49949133658&partnerID=8YFLogxK

M3 - Article

VL - 1

SP - 159

EP - 164

JO - BioSystems

JF - BioSystems

SN - 0303-2647

IS - 3

ER -