Contractile and metabolic properties of engineered skeletal muscle derived from slow and fast phenotype mouse muscle

Alastair Khodabukus, Keith Baar

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Satellite cells derived from fast and slow muscles have been shown to adopt contractile and metabolic properties of their parent muscle. Mouse muscle shows less distinctive fiber-type profiles than rat or rabbit muscle. Therefore, in this study we sought to determine whether three-dimensional muscle constructs engineered from slow soleus (SOL) and fast tibialis anterior (TA) from mice would adopt the contractile and metabolic properties of their parent muscle. Time-to-peak tension (TPT) and half-relaxation time (1/2RT) was significantly slower in SOL constructs. In agreement with TPT, TA constructs contained significantly higher levels of fast myosin heavy chain (MHC) and fast troponin C, I, and T isoforms. Fast SERCA protein, both slow and fast calsequestrin isoforms and parvalbumin were found at higher levels in TA constructs. SOL constructs were more fatigue resistant and contained higher levels of the mitochondrial proteins SDH and ATP synthase and the fatty acid transporter CPT-1. SOL constructs contained lower levels of the glycolytic enzyme phosphofructokinase but higher levels of the β-oxidation enzymes LCAD and VLCAD suggesting greater fat oxidation. Despite no changes in PGC-1α protein, SOL constructs contained higher levels of SIRT1 and PRC. TA constructs contained higher levels of the slow-fiber program repressor SOX6 and the six transcriptional complex (STC) proteins Eya1and Six4 which may underlie the higher in fast-fiber and lower slow-fiber program proteins. Overall, we have found that muscles engineered from predominantly slow and fast mouse muscle retain contractile and metabolic properties of their native muscle.

Original languageEnglish (US)
Pages (from-to)1750-1757
Number of pages8
JournalJournal of Cellular Physiology
Volume230
Issue number8
DOIs
StatePublished - Aug 1 2015

Fingerprint

Muscle
Skeletal Muscle
Phenotype
Muscles
Fibers
Protein Isoforms
Proteins
Long-Chain Acyl-CoA Dehydrogenase
Calsequestrin
Troponin C
Phosphofructokinases
Parvalbumins
Fatty Acid Synthases
Oxidation
Troponin T
Troponin I
Myosin Heavy Chains
Mitochondrial Proteins
Enzymes
Relaxation time

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Contractile and metabolic properties of engineered skeletal muscle derived from slow and fast phenotype mouse muscle. / Khodabukus, Alastair; Baar, Keith.

In: Journal of Cellular Physiology, Vol. 230, No. 8, 01.08.2015, p. 1750-1757.

Research output: Contribution to journalArticle

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