Contractile and electrophysiologic characterization of optimized self-organizing engineered heart tissue

Claus Svane Sondergaard, Grant Mathews, Lianguo Wang, Angela Jeffreys, Amrit Sahota, Moira Wood, Crystal M Ripplinger, Ming Sing Si

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Engineered heart tissue (EHT) is being developed for clinical implantation in heart failure or congenital heart disease and therefore requires a comprehensive functional characterization and scale-up of EHT. Here we explored the effects of scale-up of self-organizing EHT and present detailed electrophysiologic and contractile functional characterization. Methods: Fibers from EHT were generated from self-organizing neonatal rat cardiac cells (0.5 × 106 to 3 × 106/fiber) on fibrin. We characterized contractile patterns and measured contractile function using a force transducer, and assessed force-length relationship, maximal force generation, and rate of force generation. Action potential and conduction velocity of EHT were measured with optical mapping, and transcript levels of myosin heavy chain beta were measured by reverse transcriptase-polymerase chain reaction. Results: Increasing the cell number per construct resulted in an increase in fiber volume. The force-length relationship was negatively impacted by increasing cell number. Maximal force generation and rate of force generation were also abrogated with increasing cell number. This decrease was not likely attributable to a selective expansion of noncontractile cells as myosin heavy chain beta levels were stable. Irregular contractile behavior was more prevalent in constructs with more cells. Engineered heart tissue (1 × 10 6/construct) had an action potential duration of 140.2 milliseconds and a conduction velocity of 23.2 cm/s. Conclusions: Engineered heart tissue displays physiologically relevant features shared with native myocardium. Engineered heart tissue scale-up by increasing cell number abrogates contractile function, possibly as a result of suboptimal cardiomyocyte performance in the absence of vasculature. Finally, conduction velocity approaches that of native myocardium without any electrical or mechanical conditioning, suggesting that the self-organizing method may be superior to other rigid scaffold-based EHT.

Original languageEnglish (US)
Pages (from-to)1241-1249
Number of pages9
JournalAnnals of Thoracic Surgery
Volume94
Issue number4
DOIs
StatePublished - Oct 2012

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Cell Count
Myosin Heavy Chains
Action Potentials
Myocardium
Fibrin
Reverse Transcriptase Polymerase Chain Reaction
Transducers
Cardiac Myocytes
Heart Diseases
Heart Failure

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery
  • Pulmonary and Respiratory Medicine

Cite this

Sondergaard, C. S., Mathews, G., Wang, L., Jeffreys, A., Sahota, A., Wood, M., ... Si, M. S. (2012). Contractile and electrophysiologic characterization of optimized self-organizing engineered heart tissue. Annals of Thoracic Surgery, 94(4), 1241-1249. https://doi.org/10.1016/j.athoracsur.2012.04.098

Contractile and electrophysiologic characterization of optimized self-organizing engineered heart tissue. / Sondergaard, Claus Svane; Mathews, Grant; Wang, Lianguo; Jeffreys, Angela; Sahota, Amrit; Wood, Moira; Ripplinger, Crystal M; Si, Ming Sing.

In: Annals of Thoracic Surgery, Vol. 94, No. 4, 10.2012, p. 1241-1249.

Research output: Contribution to journalArticle

Sondergaard, Claus Svane ; Mathews, Grant ; Wang, Lianguo ; Jeffreys, Angela ; Sahota, Amrit ; Wood, Moira ; Ripplinger, Crystal M ; Si, Ming Sing. / Contractile and electrophysiologic characterization of optimized self-organizing engineered heart tissue. In: Annals of Thoracic Surgery. 2012 ; Vol. 94, No. 4. pp. 1241-1249.
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