Contraception in mares heteroimmunized with pig zonae pellucidae

Irwin Liu, M. Bernoco, M. Feldman

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Ten fertile feral mares and 6 domestic horses (4 fertile mares, 1 infertile mare, 1 gelding) were immunized with heat-solubilized pig zonae pellucidae by 4 injections equivalent to 2000 or 5000 zonae each at 2-4-week intervals and a booster injection of 20000 zonae 6-10 months after the last of the initial inoculations. The immune response was reflected by high antibody levels as measured by an enzyme-linked immunosorbent assay (ELISA) using immobilized pig zona antigen. In-vivo inhibition of fertility occurred in 12 (86%) of the 14 fertile mares studied and persisted for a minimum of 7 months. Repeated mating of the fertile domestic mares resulted in conception when anti-pig zona antibody concentrations had decreased from initial peak absorbance ratios (>1.0) to relatively lower levels (0.64 or less with one exception). An indirect immunofluorescence assay, revealed a considerably lower cross-reactive antibody titre with horse oocytes as compared to pig oocytes. Clinical, endocrinological and histological analyses of the ovaries and their function following regained fertility after immunization revealed no abnormalities. One mare remained infertile.

Original languageEnglish (US)
Pages (from-to)19-29
Number of pages11
JournalJournal of Reproduction and Fertility
Volume85
Issue number1
DOIs
StatePublished - Jan 1 1989

Fingerprint

Zona Pellucida
Herpes Zoster
Contraception
Swine
Horses
Oocytes
Fertility
Antibodies
Injections
Indirect Fluorescent Antibody Technique
Ovary
Immunization
Hot Temperature
Enzyme-Linked Immunosorbent Assay
Antigens

ASJC Scopus subject areas

  • Physiology
  • Embryology
  • Molecular Biology
  • Obstetrics and Gynecology
  • Developmental Biology

Cite this

Contraception in mares heteroimmunized with pig zonae pellucidae. / Liu, Irwin; Bernoco, M.; Feldman, M.

In: Journal of Reproduction and Fertility, Vol. 85, No. 1, 01.01.1989, p. 19-29.

Research output: Contribution to journalArticle

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