Continuously delivered ovarian steroids do not alter dendritic spine density or morphology in macaque dorsolateral prefrontal cortical neurons

M. E. Young, D. T. Ohm, W. G.M. Janssen, N. A. Gee, B. L. Lasley, John Morrison

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aged ovariectomized (OVX) female monkeys, a model for menopause in humans, show a decline in spine density in the dorsolateral prefrontal cortex (dlPFC) and diminished performance in cognitive tasks requiring this brain region. Previous studies in our laboratory have shown that long-term cyclic treatment with 17β-estradiol (E) produces an increase in spine density and in the proportion of thinner spines in layer III pyramidal neurons in the dlPFC of both young and aged OVX rhesus monkeys. Here we used 3D reconstruction of Lucifer yellow-loaded neurons to investigate whether clinically relevant schedules of hormone therapy would produce similar changes in prefrontal cortical neuronal morphology as long-term cyclic E treatment in young female monkeys. We found that continuously delivered E, with or without a cyclic progesterone treatment, did not alter spine density or morphology in the dlPFC of young adult OVX rhesus monkeys. We also found that the increased density of thinner spines evident in the dlPFC 24. h after E administration in the context of long-term cyclic E therapy is no longer detectable 20. days after E treatment. When compared with the results of our previously published investigations, our results suggest that cyclic fluctuations in serum E levels may cause corresponding fluctuations in the density of thin spines in the dlPFC. By contrast, continuous administration of E does not support sustained increases in thin spine density. Physiological fluctuations in E concentration may be necessary to maintain the morphological sensitivity of the dlPFC to E.

Original languageEnglish (US)
Pages (from-to)219-225
Number of pages7
JournalNeuroscience
Volume255
DOIs
StatePublished - Dec 26 2013
Externally publishedYes

Fingerprint

Dendritic Spines
Macaca
Prefrontal Cortex
Spine
Steroids
Neurons
Macaca mulatta
Haplorhini
Therapeutics
Pyramidal Cells
Menopause
Progesterone
Young Adult
Estradiol
Appointments and Schedules
Hormones
Brain
Serum

Keywords

  • Aging
  • Estrogen
  • Hormone replacement therapy
  • Prefrontal cortex
  • Primate
  • Progesterone

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Continuously delivered ovarian steroids do not alter dendritic spine density or morphology in macaque dorsolateral prefrontal cortical neurons. / Young, M. E.; Ohm, D. T.; Janssen, W. G.M.; Gee, N. A.; Lasley, B. L.; Morrison, John.

In: Neuroscience, Vol. 255, 26.12.2013, p. 219-225.

Research output: Contribution to journalArticle

Young, M. E. ; Ohm, D. T. ; Janssen, W. G.M. ; Gee, N. A. ; Lasley, B. L. ; Morrison, John. / Continuously delivered ovarian steroids do not alter dendritic spine density or morphology in macaque dorsolateral prefrontal cortical neurons. In: Neuroscience. 2013 ; Vol. 255. pp. 219-225.
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