Abstract
Using non-lithographic fabrication methods developed in our laboratory, we prepared continuous-flow microfluidic analyzers. We tested these devices in experiments involving detection of bacterial endospores. The detection was based on the enhancement of the fluorescence of a cationic dye, 3,3′- diethylthiacyanine iodide (THIA), in the presence of spores. We were able to detect as few as ∼10 5 spores when injected in a device. The measurements with the micro-fluidic devices manifested significantly higher sensitivity for bacterial spores than for vegetative bacteria. Such distinction between spores and vegetative bacteria could not be achieved with THIA using steady-state emission measurements.
| Original language | English (US) |
|---|---|
| Title of host publication | Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06 |
| Volume | 2006 |
| State | Published - 2006 |
| Event | Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06 - Irvine, CA, United States Duration: Jun 8 2006 → Jun 9 2006 |
Other
| Other | Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06 |
|---|---|
| Country | United States |
| City | Irvine, CA |
| Period | 6/8/06 → 6/9/06 |
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ASJC Scopus subject areas
- Engineering(all)
Cite this
Continuous flow microfluidic devices for detection of bacterial endospores. / Wan, Jiandi; Landsman, Pavel; Bing, Xia; Bower, Paul E.; Heinrich, Volkmar; Jones, Guilford; Vullev, Valentine I.
Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06. Vol. 2006 2006.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Continuous flow microfluidic devices for detection of bacterial endospores
AU - Wan, Jiandi
AU - Landsman, Pavel
AU - Bing, Xia
AU - Bower, Paul E.
AU - Heinrich, Volkmar
AU - Jones, Guilford
AU - Vullev, Valentine I.
PY - 2006
Y1 - 2006
N2 - Using non-lithographic fabrication methods developed in our laboratory, we prepared continuous-flow microfluidic analyzers. We tested these devices in experiments involving detection of bacterial endospores. The detection was based on the enhancement of the fluorescence of a cationic dye, 3,3′- diethylthiacyanine iodide (THIA), in the presence of spores. We were able to detect as few as ∼10 5 spores when injected in a device. The measurements with the micro-fluidic devices manifested significantly higher sensitivity for bacterial spores than for vegetative bacteria. Such distinction between spores and vegetative bacteria could not be achieved with THIA using steady-state emission measurements.
AB - Using non-lithographic fabrication methods developed in our laboratory, we prepared continuous-flow microfluidic analyzers. We tested these devices in experiments involving detection of bacterial endospores. The detection was based on the enhancement of the fluorescence of a cationic dye, 3,3′- diethylthiacyanine iodide (THIA), in the presence of spores. We were able to detect as few as ∼10 5 spores when injected in a device. The measurements with the micro-fluidic devices manifested significantly higher sensitivity for bacterial spores than for vegetative bacteria. Such distinction between spores and vegetative bacteria could not be achieved with THIA using steady-state emission measurements.
UR - http://www.scopus.com/inward/record.url?scp=33845759035&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845759035&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:33845759035
VL - 2006
BT - Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06
ER -