Continuous flow microfluidic devices for detection of bacterial endospores

Jiandi Wan, Pavel Landsman, Xia Bing, Paul E. Bower, Volkmar Heinrich, Guilford Jones, Valentine I. Vullev

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Using non-lithographic fabrication methods developed in our laboratory, we prepared continuous-flow microfluidic analyzers. We tested these devices in experiments involving detection of bacterial endospores. The detection was based on the enhancement of the fluorescence of a cationic dye, 3,3′- diethylthiacyanine iodide (THIA), in the presence of spores. We were able to detect as few as ∼10 5 spores when injected in a device. The measurements with the micro-fluidic devices manifested significantly higher sensitivity for bacterial spores than for vegetative bacteria. Such distinction between spores and vegetative bacteria could not be achieved with THIA using steady-state emission measurements.

Original languageEnglish (US)
Title of host publicationProceedings of Frontiers in Biomedical Devices Conference, NanoBio06
Volume2006
StatePublished - 2006
EventProceedings of Frontiers in Biomedical Devices Conference, NanoBio06 - Irvine, CA, United States
Duration: Jun 8 2006Jun 9 2006

Other

OtherProceedings of Frontiers in Biomedical Devices Conference, NanoBio06
CountryUnited States
CityIrvine, CA
Period6/8/066/9/06

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Wan, J., Landsman, P., Bing, X., Bower, P. E., Heinrich, V., Jones, G., & Vullev, V. I. (2006). Continuous flow microfluidic devices for detection of bacterial endospores. In Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06 (Vol. 2006)