Abstract
Using non-lithographic fabrication methods developed in our laboratory, we prepared continuous-flow microfluidic analyzers. We tested these devices in experiments involving detection of bacterial endospores. The detection was based on the enhancement of the fluorescence of a cationic dye, 3,3′- diethylthiacyanine iodide (THIA), in the presence of spores. We were able to detect as few as ∼10 5 spores when injected in a device. The measurements with the micro-fluidic devices manifested significantly higher sensitivity for bacterial spores than for vegetative bacteria. Such distinction between spores and vegetative bacteria could not be achieved with THIA using steady-state emission measurements.
Original language | English (US) |
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Title of host publication | Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06 |
Volume | 2006 |
State | Published - 2006 |
Event | Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06 - Irvine, CA, United States Duration: Jun 8 2006 → Jun 9 2006 |
Other
Other | Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06 |
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Country | United States |
City | Irvine, CA |
Period | 6/8/06 → 6/9/06 |
ASJC Scopus subject areas
- Engineering(all)