Continuous flow microfluidic devices for detection of bacterial endospores

Jiandi Wan; Pavel Landsman; Xia Bing; Paul E. Bower; Volkmar Heinrich; Guilford Jones; Valentine I. Vullev

Continuous flow microfluidic devices for detection of bacterial endospores

Jiandi Wan, Pavel Landsman, Xia Bing, Paul E. Bower, Volkmar Heinrich, Guilford Jones, Valentine I. Vullev

Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Using non-lithographic fabrication methods developed in our laboratory, we prepared continuous-flow microfluidic analyzers. We tested these devices in experiments involving detection of bacterial endospores. The detection was based on the enhancement of the fluorescence of a cationic dye, 3,3′- diethylthiacyanine iodide (THIA), in the presence of spores. We were able to detect as few as ∼10 ⁵ spores when injected in a device. The measurements with the micro-fluidic devices manifested significantly higher sensitivity for bacterial spores than for vegetative bacteria. Such distinction between spores and vegetative bacteria could not be achieved with THIA using steady-state emission measurements.

Original language	English (US)
Title of host publication	Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06
Volume	2006
State	Published - 2006
Event	Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06 - Irvine, CA, United States Duration: Jun 8 2006 → Jun 9 2006

Other

Other	Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06
Country	United States
City	Irvine, CA
Period	6/8/06 → 6/9/06

ASJC Scopus subject areas

Engineering(all)

Access to Document

Fingerprint Dive into the research topics of 'Continuous flow microfluidic devices for detection of bacterial endospores'. Together they form a unique fingerprint.

Cite this

Wan, J., Landsman, P., Bing, X., Bower, P. E., Heinrich, V., Jones, G., & Vullev, V. I. (2006). Continuous flow microfluidic devices for detection of bacterial endospores. In Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06 (Vol. 2006)

@inproceedings{fbbecdcc308f4524826cb59fc2b3a131,

title = "Continuous flow microfluidic devices for detection of bacterial endospores",

abstract = "Using non-lithographic fabrication methods developed in our laboratory, we prepared continuous-flow microfluidic analyzers. We tested these devices in experiments involving detection of bacterial endospores. The detection was based on the enhancement of the fluorescence of a cationic dye, 3,3′- diethylthiacyanine iodide (THIA), in the presence of spores. We were able to detect as few as ∼10 5 spores when injected in a device. The measurements with the micro-fluidic devices manifested significantly higher sensitivity for bacterial spores than for vegetative bacteria. Such distinction between spores and vegetative bacteria could not be achieved with THIA using steady-state emission measurements.",

author = "Jiandi Wan and Pavel Landsman and Xia Bing and Bower, {Paul E.} and Volkmar Heinrich and Guilford Jones and Vullev, {Valentine I.}",

year = "2006",

language = "English (US)",

volume = "2006",

booktitle = "Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06",

note = "Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06 ; Conference date: 08-06-2006 Through 09-06-2006",

}

TY - GEN

T1 - Continuous flow microfluidic devices for detection of bacterial endospores

AU - Wan, Jiandi

AU - Landsman, Pavel

AU - Bing, Xia

AU - Bower, Paul E.

AU - Heinrich, Volkmar

AU - Jones, Guilford

AU - Vullev, Valentine I.

PY - 2006

Y1 - 2006

N2 - Using non-lithographic fabrication methods developed in our laboratory, we prepared continuous-flow microfluidic analyzers. We tested these devices in experiments involving detection of bacterial endospores. The detection was based on the enhancement of the fluorescence of a cationic dye, 3,3′- diethylthiacyanine iodide (THIA), in the presence of spores. We were able to detect as few as ∼10 5 spores when injected in a device. The measurements with the micro-fluidic devices manifested significantly higher sensitivity for bacterial spores than for vegetative bacteria. Such distinction between spores and vegetative bacteria could not be achieved with THIA using steady-state emission measurements.

AB - Using non-lithographic fabrication methods developed in our laboratory, we prepared continuous-flow microfluidic analyzers. We tested these devices in experiments involving detection of bacterial endospores. The detection was based on the enhancement of the fluorescence of a cationic dye, 3,3′- diethylthiacyanine iodide (THIA), in the presence of spores. We were able to detect as few as ∼10 5 spores when injected in a device. The measurements with the micro-fluidic devices manifested significantly higher sensitivity for bacterial spores than for vegetative bacteria. Such distinction between spores and vegetative bacteria could not be achieved with THIA using steady-state emission measurements.

UR - http://www.scopus.com/inward/record.url?scp=33845759035&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845759035&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:33845759035

VL - 2006

BT - Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06

T2 - Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06

Y2 - 8 June 2006 through 9 June 2006

ER -