Continuous flow microfluidic devices for detection of bacterial endospores

Jiandi Wan, Pavel Landsman, Xia Bing, Paul E. Bower, Volkmar Heinrich, Guilford Jones, Valentine I. Vullev

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Using non-lithographic fabrication methods developed in our laboratory, we prepared continuous-flow microfluidic analyzers. We tested these devices in experiments involving detection of bacterial endospores. The detection was based on the enhancement of the fluorescence of a cationic dye, 3,3′- diethylthiacyanine iodide (THIA), in the presence of spores. We were able to detect as few as ∼10 5 spores when injected in a device. The measurements with the micro-fluidic devices manifested significantly higher sensitivity for bacterial spores than for vegetative bacteria. Such distinction between spores and vegetative bacteria could not be achieved with THIA using steady-state emission measurements.

Original languageEnglish (US)
Title of host publicationProceedings of Frontiers in Biomedical Devices Conference, NanoBio06
Volume2006
StatePublished - 2006
EventProceedings of Frontiers in Biomedical Devices Conference, NanoBio06 - Irvine, CA, United States
Duration: Jun 8 2006Jun 9 2006

Other

OtherProceedings of Frontiers in Biomedical Devices Conference, NanoBio06
CountryUnited States
CityIrvine, CA
Period6/8/066/9/06

Fingerprint

Microfluidics
Bacteria
Fluidic devices
Dyes
Fluorescence
Fabrication
Experiments

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Wan, J., Landsman, P., Bing, X., Bower, P. E., Heinrich, V., Jones, G., & Vullev, V. I. (2006). Continuous flow microfluidic devices for detection of bacterial endospores. In Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06 (Vol. 2006)

Continuous flow microfluidic devices for detection of bacterial endospores. / Wan, Jiandi; Landsman, Pavel; Bing, Xia; Bower, Paul E.; Heinrich, Volkmar; Jones, Guilford; Vullev, Valentine I.

Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06. Vol. 2006 2006.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wan, J, Landsman, P, Bing, X, Bower, PE, Heinrich, V, Jones, G & Vullev, VI 2006, Continuous flow microfluidic devices for detection of bacterial endospores. in Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06. vol. 2006, Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06, Irvine, CA, United States, 6/8/06.
Wan J, Landsman P, Bing X, Bower PE, Heinrich V, Jones G et al. Continuous flow microfluidic devices for detection of bacterial endospores. In Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06. Vol. 2006. 2006
Wan, Jiandi ; Landsman, Pavel ; Bing, Xia ; Bower, Paul E. ; Heinrich, Volkmar ; Jones, Guilford ; Vullev, Valentine I. / Continuous flow microfluidic devices for detection of bacterial endospores. Proceedings of Frontiers in Biomedical Devices Conference, NanoBio06. Vol. 2006 2006.
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