Abstract
Heterofunctional azide and alkyne PEG-linkers have been synthesized and site specifically conjugated to scFv via a reactive thiol functionality; two scFv were coupled by copper catalyzed 1,3-dipolar cycloaddition to make divalent scFv (di-scFv) with an inter-scFv distance defined to provide divalent binding; antigen binding was maintained for the di-scFv construct and increased several times compared to that of the parent scFv; the cycloaddition reaction reported herein represents an important ligation strategy to covalently link macromolecular proteins and retain sensitive structural conformations.
Original language | English (US) |
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Pages (from-to) | 695-697 |
Number of pages | 3 |
Journal | Chemical Communications |
Issue number | 7 |
DOIs | |
State | Published - 2007 |
ASJC Scopus subject areas
- Chemistry(all)