Abstract
Heterofunctional azide and alkyne PEG-linkers have been synthesized and site specifically conjugated to scFv via a reactive thiol functionality; two scFv were coupled by copper catalyzed 1,3-dipolar cycloaddition to make divalent scFv (di-scFv) with an inter-scFv distance defined to provide divalent binding; antigen binding was maintained for the di-scFv construct and increased several times compared to that of the parent scFv; the cycloaddition reaction reported herein represents an important ligation strategy to covalently link macromolecular proteins and retain sensitive structural conformations.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 695-697 |
| Number of pages | 3 |
| Journal | Chemical Communications |
| Issue number | 7 |
| DOIs | |
| State | Published - 2007 |
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ASJC Scopus subject areas
- Chemistry(all)
Cite this
Construction of di-scFv through a trivalent alkyne-azide 1,3-dipolar cycloaddition. / Natarajan, Arutselvan; Du, Wenjun; Xiong, Cheng Yi; Denardo, Gerald L; DeNardo, Sally J.; Gervay-Hague, Jacquelyn.
In: Chemical Communications, No. 7, 2007, p. 695-697.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Construction of di-scFv through a trivalent alkyne-azide 1,3-dipolar cycloaddition
AU - Natarajan, Arutselvan
AU - Du, Wenjun
AU - Xiong, Cheng Yi
AU - Denardo, Gerald L
AU - DeNardo, Sally J.
AU - Gervay-Hague, Jacquelyn
PY - 2007
Y1 - 2007
N2 - Heterofunctional azide and alkyne PEG-linkers have been synthesized and site specifically conjugated to scFv via a reactive thiol functionality; two scFv were coupled by copper catalyzed 1,3-dipolar cycloaddition to make divalent scFv (di-scFv) with an inter-scFv distance defined to provide divalent binding; antigen binding was maintained for the di-scFv construct and increased several times compared to that of the parent scFv; the cycloaddition reaction reported herein represents an important ligation strategy to covalently link macromolecular proteins and retain sensitive structural conformations.
AB - Heterofunctional azide and alkyne PEG-linkers have been synthesized and site specifically conjugated to scFv via a reactive thiol functionality; two scFv were coupled by copper catalyzed 1,3-dipolar cycloaddition to make divalent scFv (di-scFv) with an inter-scFv distance defined to provide divalent binding; antigen binding was maintained for the di-scFv construct and increased several times compared to that of the parent scFv; the cycloaddition reaction reported herein represents an important ligation strategy to covalently link macromolecular proteins and retain sensitive structural conformations.
UR - http://www.scopus.com/inward/record.url?scp=33846902068&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846902068&partnerID=8YFLogxK
U2 - 10.1039/b611636a
DO - 10.1039/b611636a
M3 - Article
C2 - 17392953
AN - SCOPUS:33846902068
SP - 695
EP - 697
JO - Chemical Communications
JF - Chemical Communications
SN - 1359-7345
IS - 7
ER -