Abstract
Heterofunctional azide and alkyne PEG-linkers have been synthesized and site specifically conjugated to scFv via a reactive thiol functionality; two scFv were coupled by copper catalyzed 1,3-dipolar cycloaddition to make divalent scFv (di-scFv) with an inter-scFv distance defined to provide divalent binding; antigen binding was maintained for the di-scFv construct and increased several times compared to that of the parent scFv; the cycloaddition reaction reported herein represents an important ligation strategy to covalently link macromolecular proteins and retain sensitive structural conformations.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 695-697 |
| Number of pages | 3 |
| Journal | Chemical Communications |
| Issue number | 7 |
| DOIs | |
| State | Published - 2007 |
ASJC Scopus subject areas
- Chemistry(all)
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Natarajan, A., Du, W., Xiong, C. Y., Denardo, G. L., DeNardo, S. J., & Gervay-Hague, J. (2007). Construction of di-scFv through a trivalent alkyne-azide 1,3-dipolar cycloaddition. Chemical Communications, (7), 695-697. https://doi.org/10.1039/b611636a