Construction of di-scFv through a trivalent alkyne-azide 1,3-dipolar cycloaddition

Arutselvan Natarajan, Wenjun Du, Cheng Yi Xiong, Gerald L Denardo, Sally J. DeNardo, Jacquelyn Gervay-Hague

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Heterofunctional azide and alkyne PEG-linkers have been synthesized and site specifically conjugated to scFv via a reactive thiol functionality; two scFv were coupled by copper catalyzed 1,3-dipolar cycloaddition to make divalent scFv (di-scFv) with an inter-scFv distance defined to provide divalent binding; antigen binding was maintained for the di-scFv construct and increased several times compared to that of the parent scFv; the cycloaddition reaction reported herein represents an important ligation strategy to covalently link macromolecular proteins and retain sensitive structural conformations.

Original languageEnglish (US)
Pages (from-to)695-697
Number of pages3
JournalChemical Communications
Issue number7
DOIs
StatePublished - 2007

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Alkynes
Azides
Cycloaddition
Antigens
Sulfhydryl Compounds
Polyethylene glycols
Conformations
Copper
Proteins

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Construction of di-scFv through a trivalent alkyne-azide 1,3-dipolar cycloaddition. / Natarajan, Arutselvan; Du, Wenjun; Xiong, Cheng Yi; Denardo, Gerald L; DeNardo, Sally J.; Gervay-Hague, Jacquelyn.

In: Chemical Communications, No. 7, 2007, p. 695-697.

Research output: Contribution to journalArticle

Natarajan, Arutselvan ; Du, Wenjun ; Xiong, Cheng Yi ; Denardo, Gerald L ; DeNardo, Sally J. ; Gervay-Hague, Jacquelyn. / Construction of di-scFv through a trivalent alkyne-azide 1,3-dipolar cycloaddition. In: Chemical Communications. 2007 ; No. 7. pp. 695-697.
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