Construction of di-scFv through a trivalent alkyne-azide 1,3-dipolar cycloaddition

Arutselvan Natarajan; Wenjun Du; Cheng Yi Xiong; Gerald L Denardo; Sally J. DeNardo; Jacquelyn Gervay-Hague

doi:10.1039/b611636a

Construction of di-scFv through a trivalent alkyne-azide 1,3-dipolar cycloaddition

Arutselvan Natarajan, Wenjun Du, Cheng Yi Xiong, Gerald L Denardo, Sally J. DeNardo, Jacquelyn Gervay-Hague

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

Heterofunctional azide and alkyne PEG-linkers have been synthesized and site specifically conjugated to scFv via a reactive thiol functionality; two scFv were coupled by copper catalyzed 1,3-dipolar cycloaddition to make divalent scFv (di-scFv) with an inter-scFv distance defined to provide divalent binding; antigen binding was maintained for the di-scFv construct and increased several times compared to that of the parent scFv; the cycloaddition reaction reported herein represents an important ligation strategy to covalently link macromolecular proteins and retain sensitive structural conformations.

Original language	English (US)
Pages (from-to)	695-697
Number of pages	3
Journal	Chemical Communications
Issue number	7
DOIs	https://doi.org/10.1039/b611636a
State	Published - 2007

ASJC Scopus subject areas

Chemistry(all)

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abstract = "Heterofunctional azide and alkyne PEG-linkers have been synthesized and site specifically conjugated to scFv via a reactive thiol functionality; two scFv were coupled by copper catalyzed 1,3-dipolar cycloaddition to make divalent scFv (di-scFv) with an inter-scFv distance defined to provide divalent binding; antigen binding was maintained for the di-scFv construct and increased several times compared to that of the parent scFv; the cycloaddition reaction reported herein represents an important ligation strategy to covalently link macromolecular proteins and retain sensitive structural conformations.",

author = "Arutselvan Natarajan and Wenjun Du and Xiong, {Cheng Yi} and Denardo, {Gerald L} and DeNardo, {Sally J.} and Jacquelyn Gervay-Hague",

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doi = "10.1039/b611636a",

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AU - Natarajan, Arutselvan

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AU - Xiong, Cheng Yi

AU - Denardo, Gerald L

AU - DeNardo, Sally J.

AU - Gervay-Hague, Jacquelyn

PY - 2007

Y1 - 2007

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AB - Heterofunctional azide and alkyne PEG-linkers have been synthesized and site specifically conjugated to scFv via a reactive thiol functionality; two scFv were coupled by copper catalyzed 1,3-dipolar cycloaddition to make divalent scFv (di-scFv) with an inter-scFv distance defined to provide divalent binding; antigen binding was maintained for the di-scFv construct and increased several times compared to that of the parent scFv; the cycloaddition reaction reported herein represents an important ligation strategy to covalently link macromolecular proteins and retain sensitive structural conformations.

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Construction of di-scFv through a trivalent alkyne-azide 1,3-dipolar cycloaddition

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