Construction of an infectious HIV type 1 molecular clone from an African patient with a subtype D/C recombinant virus

Binshan Shi, Sean M. Philpott, Barbara Weiser, Carla Kuiken, Cheryl Brunner, Guowei Fang, Keith R. Fowke, Francis A. Plummer, Sarah Rowland-Jones, Job Bwayo, Aggrey O. Anzala, Joshua Kimani, Rupert Kaul, Harold Burger

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The majority of HIV-1 infections worldwide occur in Africa, where subtype B viruses are rare and intersubtype recombinants are common. Pathogenesis and vaccine studies need to focus on viruses derived from African patients, and infectious HIV-1 molecular clones can be useful tools. To clone non-B subtypes and recombinant viruses from patients, we cultivated HIV-1 from the plasma of a Kenyan long-term survivor. Viral DNA was cloned into a plasmid, which was transfected into COS cells; progeny virus was propagated in PBMCs. Sequence analyses revealed that both the patient's plasma HIV-1 RNA and the cloned DNA genomes were recombinants between subtypes D and C; subtype C sequences comprised the nef and LTR regions. The cloned virus used the CCR5 coreceptor and did not form syncytia in vitro. This infectious HIV-1 subtype D/C recombinant molecular clone obtained from a Kenyan long-term survivor promises to be useful to study pathogenesis and vaccine design.

Original languageEnglish (US)
Pages (from-to)1015-1018
Number of pages4
JournalAIDS Research and Human Retroviruses
Volume20
Issue number9
DOIs
StatePublished - Sep 2004
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Shi, B., Philpott, S. M., Weiser, B., Kuiken, C., Brunner, C., Fang, G., Fowke, K. R., Plummer, F. A., Rowland-Jones, S., Bwayo, J., Anzala, A. O., Kimani, J., Kaul, R., & Burger, H. (2004). Construction of an infectious HIV type 1 molecular clone from an African patient with a subtype D/C recombinant virus. AIDS Research and Human Retroviruses, 20(9), 1015-1018. https://doi.org/10.1089/aid.2004.20.1015