Abstract
Creation of large (∼ 15 kb) recombinant plasmids can be done in a single step by co-transformation of yeast cells with a partial restriction digest of a plasmid vector and a linear insert whose ends overlap one of the vector restriction sites. This method is used to generate a plasmid expressing the Saccharomyces cerevisiae rRNA genes containing the Ca.LSU group 1 intron ribozyme from Candida albicans. This plasmid expresses functional rRNA and ribozyme.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1527-1534 |
| Number of pages | 8 |
| Journal | Yeast |
| Volume | 16 |
| Issue number | 16 |
| DOIs | |
| State | Published - 2000 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biotechnology
- Bioengineering
- Microbiology
Cite this
Construction of a large plasmid lacking linearizing single restriction sites by simultaneous in vivo recombination and plasmid shuffling in yeast. / Miletti, Karl E.; Leibowitz, Michael J.
In: Yeast, Vol. 16, No. 16, 2000, p. 1527-1534.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Construction of a large plasmid lacking linearizing single restriction sites by simultaneous in vivo recombination and plasmid shuffling in yeast
AU - Miletti, Karl E.
AU - Leibowitz, Michael J
PY - 2000
Y1 - 2000
N2 - Creation of large (∼ 15 kb) recombinant plasmids can be done in a single step by co-transformation of yeast cells with a partial restriction digest of a plasmid vector and a linear insert whose ends overlap one of the vector restriction sites. This method is used to generate a plasmid expressing the Saccharomyces cerevisiae rRNA genes containing the Ca.LSU group 1 intron ribozyme from Candida albicans. This plasmid expresses functional rRNA and ribozyme.
AB - Creation of large (∼ 15 kb) recombinant plasmids can be done in a single step by co-transformation of yeast cells with a partial restriction digest of a plasmid vector and a linear insert whose ends overlap one of the vector restriction sites. This method is used to generate a plasmid expressing the Saccharomyces cerevisiae rRNA genes containing the Ca.LSU group 1 intron ribozyme from Candida albicans. This plasmid expresses functional rRNA and ribozyme.
UR - http://www.scopus.com/inward/record.url?scp=0034532992&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034532992&partnerID=8YFLogxK
U2 - 10.1002/1097-0061(200012)16:16<1527::AID-YEA643>3.0.CO;2-8
DO - 10.1002/1097-0061(200012)16:16<1527::AID-YEA643>3.0.CO;2-8
M3 - Article
C2 - 11113975
AN - SCOPUS:0034532992
VL - 16
SP - 1527
EP - 1534
JO - Yeast
JF - Yeast
SN - 0749-503X
IS - 16
ER -