Abstract
Creation of large (∼ 15 kb) recombinant plasmids can be done in a single step by co-transformation of yeast cells with a partial restriction digest of a plasmid vector and a linear insert whose ends overlap one of the vector restriction sites. This method is used to generate a plasmid expressing the Saccharomyces cerevisiae rRNA genes containing the Ca.LSU group 1 intron ribozyme from Candida albicans. This plasmid expresses functional rRNA and ribozyme.
Original language | English (US) |
---|---|
Pages (from-to) | 1527-1534 |
Number of pages | 8 |
Journal | Yeast |
Volume | 16 |
Issue number | 16 |
DOIs | |
State | Published - 2000 |
Externally published | Yes |
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biotechnology
- Bioengineering
- Microbiology